http://hdl.handle.net/2027.42/58662 Tue, 21 May 2013 17:37:55 GMT 2013-05-21T17:37:55Z http://deepblue.lib.umich.edu:80/bitstream/id/213632/pharmacy.gif http://hdl.handle.net/2027.42/58662 http://hdl.handle.net/2027.42/84659 Bioaccumulation and Biodistribution Knowledgebase Rosania, Gus R Mon, 13 Jun 2011 00:00:00 GMT http://hdl.handle.net/2027.42/84659 2011-06-13T00:00:00Z http://hdl.handle.net/2027.42/83255 Atomic Physicochemical Parameters for Three Dimensional Structure Directed Quantitative Structure-Activity Relationships II. Modeling Dispersive and Hydrophobic Interactions Ghose, Arup K.; Crippen, Gordon M. Thu, 01 Jan 1987 00:00:00 GMT http://hdl.handle.net/2027.42/83255 1987-01-01T00:00:00Z http://hdl.handle.net/2027.42/60122 HeLa cells incubated with styryl compound H168 Rosania, Gus; Shedden, Kerby This is part of a larger collection of archives of 1344 styryl compounds containing digital microscopic images of HeLa cells incubated with styryl compounds, along with other information about those compounds. ** The compounds were synthesized in a combinatorial synthesis involving 8 pyridinium/quinolinium groups (A-H) and 168 aldehyde groups (1-168). The compounds are identified by their A-H letter followed by their 1-168 number. ** Each of the 12 images in this archive is a 512x512 pixel image stored as a bzip2 compressed sequence of 2 byte unsigned short integers (little endian format), in standard raster order. ** Fluorescence in the Hoechst channel derives from Hoechst dye. Fluorescence in the other channels is presumed to derive from the styryl molecules, although cellular autofluorescence is also present. ** The images were acquired at 200X magnification with a 20X objective. The images were acquired with a 12 bit CCD camera so the greyscale intensities range from 0 to 4095. ** The "withdye" images were acquired after around 1 hour of incubation with the styryl probe. Next the probe was washed out using an onboard robotic pipetting system, and the "washout" images were acquired. ** The Hoechst dye images were acquired at 50msec exposure time, the other channels were acquired at both a 1sec and 200msec exposure time (Cy5 was only acquired at 1sec). ** The images were obtained using a Cellomics KineticScan instrument. Cells were kept alive and healthy in an onboard environmental control chamber. ** Styryl compounds were present at 100 micromolar concentration. Tue, 01 Jan 2008 00:00:00 GMT http://hdl.handle.net/2027.42/60122 2008-01-01T00:00:00Z http://hdl.handle.net/2027.42/60121 HeLa cells incubated with styryl compound H167 Rosania, Gus; Shedden, Kerby This is part of a larger collection of archives of 1344 styryl compounds containing digital microscopic images of HeLa cells incubated with styryl compounds, along with other information about those compounds. ** The compounds were synthesized in a combinatorial synthesis involving 8 pyridinium/quinolinium groups (A-H) and 168 aldehyde groups (1-168). The compounds are identified by their A-H letter followed by their 1-168 number. ** Each of the 12 images in this archive is a 512x512 pixel image stored as a bzip2 compressed sequence of 2 byte unsigned short integers (little endian format), in standard raster order. ** Fluorescence in the Hoechst channel derives from Hoechst dye. Fluorescence in the other channels is presumed to derive from the styryl molecules, although cellular autofluorescence is also present. ** The images were acquired at 200X magnification with a 20X objective. The images were acquired with a 12 bit CCD camera so the greyscale intensities range from 0 to 4095. ** The "withdye" images were acquired after around 1 hour of incubation with the styryl probe. Next the probe was washed out using an onboard robotic pipetting system, and the "washout" images were acquired. ** The Hoechst dye images were acquired at 50msec exposure time, the other channels were acquired at both a 1sec and 200msec exposure time (Cy5 was only acquired at 1sec). ** The images were obtained using a Cellomics KineticScan instrument. Cells were kept alive and healthy in an onboard environmental control chamber. ** Styryl compounds were present at 100 micromolar concentration. Tue, 01 Jan 2008 00:00:00 GMT http://hdl.handle.net/2027.42/60121 2008-01-01T00:00:00Z