Strategy and rationale for urine collection protocols employed in the NEPTUNE study
dc.contributor.author | Hogan, Marie C | |
dc.contributor.author | Lieske, John C | |
dc.contributor.author | Lienczewski, Chrysta C | |
dc.contributor.author | Nesbitt, Lisa L | |
dc.contributor.author | Wickman, Larysa T | |
dc.contributor.author | Heyer, Christina M | |
dc.contributor.author | Harris, Peter C | |
dc.contributor.author | Ward, Christopher J | |
dc.contributor.author | Sundsbak, Jamie L | |
dc.contributor.author | Manganelli, Luca | |
dc.contributor.author | Ju, Wenjun | |
dc.contributor.author | Kopp, Jeffrey B | |
dc.contributor.author | Nelson, Peter J | |
dc.contributor.author | Adler, Sharon G | |
dc.contributor.author | Reich, Heather N | |
dc.contributor.author | Holzmann, Lawrence B | |
dc.contributor.author | Kretzler, Matthias | |
dc.contributor.author | Bitzer, Markus | |
dc.date.accessioned | 2015-11-18T19:01:59Z | |
dc.date.available | 2015-11-18T19:01:59Z | |
dc.date.issued | 2015-11-17 | |
dc.identifier.citation | BMC Nephrology. 2015 Nov 17;16(1):190 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/116023 | en_US |
dc.description.abstract | Abstract Background Glomerular diseases are potentially fatal, requiring aggressive interventions and close monitoring. Urine is a readily-accessible body fluid enriched in molecular signatures from the kidney and therefore particularly suited for routine clinical analysis as well as development of non-invasive biomarkers for glomerular diseases. Methods The Nephrotic Syndrome Study Network (NEPTUNE; ClinicalTrials.gov Identifier NCT01209000) is a North American multicenter collaborative consortium established to develop a translational research infrastructure for nephrotic syndrome. This includes standardized urine collections across all participating centers for the purpose of discovering non-invasive biomarkers for patients with nephrotic syndrome due to minimal change disease, focal segmental glomerulosclerosis, and membranous nephropathy. Here we describe the organization and methods of urine procurement and banking procedures in NEPTUNE. Results We discuss the rationale for urine collection and storage conditions, and demonstrate the performance of three experimental analytes (neutrophil gelatinase-associated lipocalin [NGAL], retinol binding globulin, and alpha-1 microglobulin) under these conditions with and without urine preservatives (thymol, toluene, and boric acid). We also demonstrate the quality of RNA and protein collected from the urine cellular pellet and exosomes. Conclusions The urine collection protocol in NEPTUNE allows robust detection of a wide range of proteins and RNAs from urine supernatant and pellets collected longitudinally from each patient over 5 years. Combined with the detailed clinical and histopathologic data, this provides a unique resource for exploration and validation of new or accepted markers of glomerular diseases. Trial registration ClinicalTrials.gov Identifier NCT01209000 | |
dc.title | Strategy and rationale for urine collection protocols employed in the NEPTUNE study | |
dc.type | Article | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/116023/1/12882_2015_Article_185.pdf | |
dc.identifier.doi | 10.1186/s12882-015-0185-3 | en_US |
dc.language.rfc3066 | en | |
dc.rights.holder | Hogan et al. | |
dc.date.updated | 2015-11-18T19:01:59Z | |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.