View Item 

Show simple item record

Determination of Binding Mode: Intercalation

dc.contributor.authorDedon, Peter C.
dc.date.accessioned2018-05-15T20:12:34Z
dc.date.available2018-05-15T20:12:34Z
dc.date.issued2000-02
dc.identifier.citationDedon, Peter C. (2000). "Determination of Binding Mode: Intercalation." Current Protocols in Nucleic Acid Chemistry 00(1): 8.1.1-8.1.13.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/143603
dc.description.abstractA small molecule can be assumed to bind to DNA by intercalation between base pairs if it causes lengthening and unwinding of the DNA helix and undergoes changes in its spectral properties, such as DNA‐induced hypochromism and quenching of its UV absorbance. DNA lengthening and unwinding can be determined from the change in viscosity of a solution of linear or plasmid DNA, respectively. Intercalation of a ligand can also be seen as a reduction in the UV/visible absorbance of the intercalator, as well as a shift in the absorbance maximum.
dc.publisherAcademic Press
dc.publisherWiley Periodicals, Inc.
dc.titleDetermination of Binding Mode: Intercalation
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbtoplevelEngineering
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/143603/1/cpnc0801.pdf
dc.identifier.doi10.1002/0471142700.nc0801s00
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
dc.identifier.citedreferenceCohen, G. and Eisenberg, H. 1969. Viscosity and sedimentation study of sonicated DNA‐proflavine complexes. Biopolymers 8: 45 ‐ 55.
dc.identifier.citedreferenceCohen, G.L., Bauer, W.R., Barton, J.K., and Lippard, S.J. 1979. Binding of cis ‐ and trans ‐dichlorodiammineplatinum(II) to DNA: Evidence for unwinding and shortening of the double helix. Science 203: 1014 ‐ 1016.
dc.identifier.citedreferenceLerman, L.S. 1961. Structural considerations in the interaction of DNA and acridines. J. Mol. Biol. 3: 18 ‐ 30.
dc.identifier.citedreferenceRévet, B.M., Schmir, M., and Vinograd, J. 1971. Direct determination of the superhelix density of closed circular DNA by viscometric titration. Nature 229: 10 ‐ 13.
dc.identifier.citedreferenceSatyanarayana, S., Dabrowiak, J.C., and Chaires, J.B. 1993. Tris(phenanthroline)ruthenium(II) enantiomer interactions with DNA: Mode and specificity of binding. Biochemistry 32: 2573 ‐ 2584.
dc.identifier.citedreferenceSaucier, J.‐M. 1977. Physicochemical studies on the interaction of irehdiamine A with bihelical DNA. Biochemistry 16: 5879 ‐ 5889.
dc.identifier.citedreferenceWaring, M. 1970. Variation in the supercoils in closed circular DNA by binding of antibiotics and drugs: Evidence for molecular models involving intercalation. J. Mol. Biol. 54: 247 ‐ 279.
dc.identifier.citedreferenceWilson, W.D. and Jones, R.L. 1982. Intercalation in biological systems. In Intercalation Chemistry ( M.S. Whittingham and A.J. Jacobson, eds.)pp. 445 ‐ 501. Academic Press, New York.
dc.identifier.citedreferenceYu, L., Golik, J., Harrison, R., and Dedon, P. 1994. The deoxyfucose‐anthranilate of esperamicin A1 confers intercalative DNA binding and causes a switch in the chemistry of bistranded DNA lesions. J. Am. Chem. Soc. 116: 9733 ‐ 9738.
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
cpnc0801.pdf
Size:
167.6KB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.