Show simple item record

Determination of Binding Thermodynamics

dc.contributor.authorDedon, Peter C.
dc.date.accessioned2018-05-15T20:16:05Z
dc.date.available2018-05-15T20:16:05Z
dc.date.issued2000-02
dc.identifier.citationDedon, Peter C. (2000). "Determination of Binding Thermodynamics." Current Protocols in Nucleic Acid Chemistry 00(1): 8.2.1-8.2.8.
dc.identifier.issn1934-9270
dc.identifier.issn1934-9289
dc.identifier.urihttps://hdl.handle.net/2027.42/143787
dc.description.abstractThis unit serves as a starting point for exploring the thermodynamic properties of interactions between small molecules and DNA. It covers the determination of simple, apparent association/dissociation constants. The concentration of DNA‐bound ligand and free ligand are determined and a binding constant is extracted from these data. Data gathering and curve fitting are discussed.
dc.publisherWiley Periodicals, Inc.
dc.publisherJAI Press
dc.titleDetermination of Binding Thermodynamics
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelChemical Engineering
dc.subject.hlbsecondlevelPublic Health
dc.subject.hlbsecondlevelBiological Chemistry
dc.subject.hlbsecondlevelChemistry
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.subject.hlbtoplevelEngineering
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/143787/1/cpnc0802.pdf
dc.identifier.doi10.1002/0471142700.nc0802s00
dc.identifier.sourceCurrent Protocols in Nucleic Acid Chemistry
dc.identifier.citedreferenceChaires, J.B. 1992. Application of equilibrium binding methods to elucidate the sequence specificity of antibiotic binding to DNA. In Advances in DNA Sequence Specific Agents, Vol. 1 ( L. Hurley, ed.)pp. 3 ‐ 23. JAI Press, New York.
dc.identifier.citedreferenceChaires, J.B., Dattagupta, N., and Crothers, D.M. 1982. Studies on interaction of anthracycline antibiotics and deoxyribonucleic acid: Equilibrium binding studies on interaction of daunomycin with deoxyribonucleic acid. Biochemistry 21: 3933 ‐ 3940.
dc.identifier.citedreferenceCorreia, J.J. and Chaires, J.B. 1994. Analysis of drug‐DNA binding isotherms: A Monte Carlo approach. Methods Enzymol. 240: 593 ‐ 614.
dc.identifier.citedreferenceDattagupta, N., Hogan, M., and Crothers, D.M. 1980. Interaction of netropsin and distamycin with deoxyribonucleic acid: Electric dichroism study. Biochemistry 19: 5998 ‐ 6005.
dc.identifier.citedreferenceHinton, D.M. and Bode, V.C. 1975. Ethidium bromide affinity of circular λ‐deoxyribonucleic acid determined fluorimetrically: The effect of NaCl concentration on supercoiling. J. Biol. Chem. 250: 1061 ‐ 1070.
dc.identifier.citedreferenceJohnson, M.L. and Faunt, L.M. 1992. Parameter estimation by least‐squares methods. Methods Enzymol. 210: 1 ‐ 37.
dc.identifier.citedreferenceKrishnamurthy, G., Brenowitz, M.D., and Ellestad, G.A. 1995. Salt‐dependence of calicheamicin‐DNA site‐specific interactions. Biochemistry 34: 1001 ‐ 1010.
dc.identifier.citedreferenceMcGhee, J.D. and von Hippel, P.H. 1974. Theoretical aspects of DNA‐protein interactions: Co‐operative and non‐co‐operative binding of large ligands to a one‐dimensional homogeneous lattice. J. Mol. Biol. 86: 469 ‐ 489.
dc.identifier.citedreferenceMinton, A.P. 1990. Quantitative characterization of reversible molecular associations via analytical centrifugation. Anal. Biochem. 190: 1 ‐ 6.
dc.identifier.citedreferenceRecord, M.T. Jr., Anderson, C.F., and Lohman, T.M. 1978. Thermodynamic analysis of ion effects on the binding and conformational equilibria of proteins and nucleic acids: The role of ion association or release, screening, and ion effects on water activity. Q. Rev. Biophys. 11: 103 ‐ 178.
dc.identifier.citedreferenceWaring, M.J. 1965. Complex formation between ethidium bromide and nucleic acids. J. Mol. Biol. 13: 269 ‐ 282.
dc.identifier.citedreferenceWaring, M.J., Wakelin, L.P.G., and Lee, J.S. 1975. A solvent‐partition method for measuring the binding of drugs to DNA: Application to the quinoxaline antibiotics echinomycin and triostin A. Biochim. Biophys. Acta 407: 200 ‐ 212.
dc.identifier.citedreferenceYu, L., Golik, J., Harrison, R., and Dedon, P. 1994. The deoxyfucose‐anthranilate of esperamicin A1 confers intercalative DNA binding and causes a switch in the chemistry of bistranded DNA lesions. J. Am. Chem. Soc. 116: 9733 ‐ 9738.
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.