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Effects of Psidium guajava leaf extract on secretion systems of gram‐negative enteropathogenic bacteria

dc.contributor.authorNakasone, Noboru
dc.contributor.authorOgura, Yasunori
dc.contributor.authorHiga, Naomi
dc.contributor.authorToma, Claudia
dc.contributor.authorKoizumi, Yukiko
dc.contributor.authorTakaesu, Giichi
dc.contributor.authorSuzuki, Toshihiko
dc.contributor.authorYamashiro, Tetsu
dc.date.accessioned2018-08-13T18:49:04Z
dc.date.available2019-09-04T20:15:38Zen
dc.date.issued2018-07
dc.identifier.citationNakasone, Noboru; Ogura, Yasunori; Higa, Naomi; Toma, Claudia; Koizumi, Yukiko; Takaesu, Giichi; Suzuki, Toshihiko; Yamashiro, Tetsu (2018). "Effects of Psidium guajava leaf extract on secretion systems of gram‐negative enteropathogenic bacteria." Microbiology and Immunology 62(7): 444-453.
dc.identifier.issn0385-5600
dc.identifier.issn1348-0421
dc.identifier.urihttps://hdl.handle.net/2027.42/145239
dc.description.abstractIn this study, 672 plant‐tissue extracts were screened for phytochemicals that inhibit the function of the type III secretion system (T3SS) of enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC). Among candidates examined, an extract from the leaves of Psidium guajava (guava) was found to inhibit secretion of EPEC‐secreted protein B (EspB) from EPEC and EHEC without affecting bacterial growth. Guava extract (GE) also inhibited EPEC and EHEC from adhering to, and injecting EspB into, HEp‐2 cells. GE seemed to block translocation of EspB from the bacterial cells to the culture medium. In addition, GE also inhibited the T3SS of Yersinia pseudotuberculosis and Salmonella enterica serovar Typhimurium. After exposure to GE, Y. pseudotuberculosis stopped secreting Yersinia outer proteins and was unable to induce apoptosis of mouse bone marrow‐derived macrophages. S. typhimurium exposed to GE stopped secreting Sip proteins and was unable to invade HEp‐2 cells. GE inhibited secretion of EspC, the type V secretion protein of EPEC, but not secretion of Shiga toxin 2 from EHEC. Thus, our results suggest that guava leaves contain a novel type of antimicrobial compound that could be used to treat and prevent gram‐negative enteropathogenic bacterial infections.
dc.publisherWiley Periodicals, Inc.
dc.subject.otherPsidium guajava
dc.subject.othertype III secretion system
dc.subject.otherleaf extract
dc.titleEffects of Psidium guajava leaf extract on secretion systems of gram‐negative enteropathogenic bacteria
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelMicrobiology and Immunology
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/145239/1/mim12604.pdf
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/145239/2/mim12604_am.pdf
dc.identifier.doi10.1111/1348-0421.12604
dc.identifier.sourceMicrobiology and Immunology
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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