View Item 

Show simple item record

Extracellular Trap‐Mimicking DNA‐Histone Mesostructures Synergistically Activate Dendritic Cells

dc.contributor.authorWeerappuli, Priyan D.
dc.contributor.authorLouttit, Cameron
dc.contributor.authorKojima, Taisuke
dc.contributor.authorBrennan, Luke
dc.contributor.authorYalavarthi, Srilakshmi
dc.contributor.authorXu, Yao
dc.contributor.authorOchyl, Lukasz J.
dc.contributor.authorMaeda, Midori L.
dc.contributor.authorKim, Hong Sun
dc.contributor.authorKnight, Jason S.
dc.contributor.authorTakayama, Shuichi
dc.contributor.authorMoon, James J.
dc.date.accessioned2020-01-13T15:14:24Z
dc.date.availableWITHHELD_11_MONTHS
dc.date.available2020-01-13T15:14:24Z
dc.date.issued2019-11
dc.identifier.citationWeerappuli, Priyan D.; Louttit, Cameron; Kojima, Taisuke; Brennan, Luke; Yalavarthi, Srilakshmi; Xu, Yao; Ochyl, Lukasz J.; Maeda, Midori L.; Kim, Hong Sun; Knight, Jason S.; Takayama, Shuichi; Moon, James J. (2019). "Extracellular Trap‐Mimicking DNA‐Histone Mesostructures Synergistically Activate Dendritic Cells." Advanced Healthcare Materials 8(22): n/a-n/a.
dc.identifier.issn2192-2640
dc.identifier.issn2192-2659
dc.identifier.urihttps://hdl.handle.net/2027.42/152974
dc.description.abstractExtracellular traps (ETs), such as neutrophil extracellular traps, are a physical mesh deployed by immune cells to entrap and constrain pathogens. ETs are immunogenic structures composed of DNA, histones, and an array of variable protein and peptide components. While much attention has been paid to the multifaceted function of these structures, mechanistic studies of ETs remain challenging due to their heterogeneity and complexity. Here, a novel DNA‐histone mesostructure (DHM) formed by complexation of DNA and histones into a fibrous mesh is reported. DHMs mirror the DNA‐histone structural frame of ETs and offer a facile platform for cell culture studies. It is shown that DHMs are potent activators of dendritic cells and identify both the methylation state of DHMs and physical interaction between dendritic cells and DHMs as key tuning switches for immune stimulation. Overall, the DHM platform provides a new opportunity to study the role of ETs in immune activation and pathophysiology.A novel DNA‐histone mesostructure (DHM) platform is reported, which mirrors the morphology of extracellular traps (ETs). This platform enables bottom‐up cell‐based assays to determine the role of the DNA‐histone substructure in ET‐associated phenomena. Here, DHMs are used to investigate ET‐mediated immunostimulation.
dc.publisherWiley Periodicals, Inc.
dc.subject.othermethylation
dc.subject.otherDNA
dc.subject.otherextracellular traps
dc.subject.otherhistones
dc.subject.otherimmunostimulation
dc.titleExtracellular Trap‐Mimicking DNA‐Histone Mesostructures Synergistically Activate Dendritic Cells
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelMaterials Science and Engineering
dc.subject.hlbtoplevelEngineering
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/152974/1/adhm201900926.pdf
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/152974/2/adhm201900926_am.pdf
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/152974/3/adhm201900926-sup-0001-SuppMat.pdf
dc.identifier.doi10.1002/adhm.201900926
dc.identifier.sourceAdvanced Healthcare Materials
dc.identifier.citedreferenceJ. Bonnet, M. Colotte, D. Coudy, V. Couallier, J. Portier, B. Morin, S. Tuffet, Nucleic Acids Res. 2010, 38, 1531.
dc.identifier.citedreferenceD. R. Adams, M. Toner, R. Langer, Langmuir 2008, 24, 7688.
dc.identifier.citedreferenceR. H. Pires, S. B. Felix, M. Delcea, Nanoscale 2016, 8, 14193.
dc.identifier.citedreferenceK. Takeda, S. Akira, Semin. Immunol. 2004, 16, 3.
dc.identifier.citedreferenceL. V. Collins, S. Hajizadeh, E. Holme, I. M. Jonsson, A. Tarkowski, J. Leukocyte Biol. 2004, 75, 995.
dc.identifier.citedreferenceB. Liu, Q. Du, L. Chen, G. Fu, S. Li, L. Fu, X. Zhang, C. Ma, C. Bin, Sci. Rep. 2016, 6, 23421.
dc.identifier.citedreferenceM. Krummen, S. Balkow, L. Shen, S. Heinz, C. Loquai, H. C. Probst, S. Grabbe, J. Leukocyte Biol. 2010, 88, 189.
dc.identifier.citedreferenceV. Brinkmann, B. Laube, U. Abu Abed, C. Goosmann, A. Zychlinsky, J. Visualized Exp. 2010, 36, e1724.
dc.identifier.citedreferenceM. B. Lutz, N. Kukutsch, A. L. Ogilvie, S. Rossner, F. Koch, N. Romani, G. Schuler, J. Immunol. Methods 1999, 223, 77.
dc.identifier.citedreferenceY. Nishikawa, Y. Kajiura, J. H. Lew, J. I. Kido, T. Nagata, K. Naruishi, J. Cell. Physiol. 2017, 232, 1862.
dc.identifier.citedreferenceC. H. Lim, S. S. Adav, S. K. Sze, Y. K. Choong, R. Saravanan, A. Schmidtchen, Front. Immunol. 2018, 9, 1554.
dc.identifier.citedreferenceS. Najmeh, J. Cools‐Lartigue, B. Giannias, J. Spicer, L. E. Ferri, J. Visualized Exp. 2015, 98, e52687.
dc.identifier.citedreferenceN. K. Jain, I. Roy, Protein Sci. 2009, 18, 24.
dc.identifier.citedreferenceC. J. Desmet, K. J. Ishii, Nat. Rev. Immunol. 2012, 12, 479.
dc.identifier.citedreferenceR. Barbalat, S. E. Ewald, M. L. Mouchess, G. M. Barton, Annu. Rev. Immunol. 2011, 29, 185.
dc.identifier.citedreferenceJ. Wu, Z. J. Chen, Annu. Rev. Immunol. 2014, 32, 461.
dc.identifier.citedreferenceK. Yasuda, P. Yu, C. J. Kirschning, B. Schlatter, F. Schmitz, A. Heit, S. Bauer, H. Hochrein, H. Wagner, J. Immunol. 2005, 174, 6129.
dc.identifier.citedreferenceR. Lande, J. Gregorio, V. Facchinetti, B. Chatterjee, Y.‐H. Wang, B. Homey, W. Cao, Y.‐H. Wang, B. Su, F. O. Nestle, T. Zal, I. Mellman, J.‐M. Schröder, Y.‐J. Liu, M. Gilliet, Nature 2007, 449, 564.
dc.identifier.citedreferenceJ. Tian, A. M. Avalos, S.‐Y. Mao, B. Chen, K. Senthil, H. Wu, P. Parroche, S. Drabic, D. Golenbock, C. Sirois, J. Hua, L. L. An, L. Audoly, G. La Rosa, A. Bierhaus, P. Naworth, A. Marshak‐Rothstein, M. K. Crow, K. A. Fitzgerald, E. Latz, P. A. Kiener, A. J. Coyle, Nat. Immunol. 2007, 8, 487.
dc.identifier.citedreferenceV. Brinkmann, Science 2004, 303, 1532.
dc.identifier.citedreferenceB. McDonald, R. Urrutia, B. G. Yipp, C. N. Jenne, P. Kubes, Cell Host Microbe 2012, 12, 324.
dc.identifier.citedreferenceG. Papadaki, K. Kambas, C. Choulaki, K. Vlachou, E. Drakos, G. Bertsias, K. Ritis, D. T. Boumpas, P. R. Thompson, P. Verginis, P. Sidiropoulos, Eur. J. Immunol. 2016, 46, 2542.
dc.identifier.citedreferenceC. Lood, L. P. Blanco, M. M. Purmalek, C. Carmona‐Rivera, S. S. De Ravin, C. K. Smith, H. L. Malech, J. A. Ledbetter, K. B. Elkon, M. J. Kaplan, Nat. Med. 2016, 22, 146.
dc.identifier.citedreferenceR. Khandpur, C. Carmona‐Rivera, A. Vivekanandan‐Giri, A. Gizinski, S. Yalavarthi, J. S. Knight, S. Friday, S. Li, R. M. Patel, V. Subramanian, P. Thompson, P. Chen, D. A. Fox, S. Pennathur, M. J. Kaplan, Sci. Transl. Med. 2013, 5, 178ra40.
dc.identifier.citedreferenceK. Kessenbrock, M. Krumbholz, U. Schönermarck, W. Back, W. L. Gross, Z. Werb, H.‐J. Gröne, V. Brinkmann, D. E. Jenne, Nat. Med. 2009, 15, 623.
dc.identifier.citedreferenceS. Sangaletti, C. Tripodo, C. Chiodoni, C. Guarnotta, B. Cappetti, P. Casalini, S. Piconese, M. Parenza, C. Guiducci, C. Vitali, M. P. Colombo, Blood 2012, 120, 3007.
dc.identifier.citedreferenceR. Lande, D. Ganguly, V. Facchinetti, L. Frasca, C. Conrad, J. Gregorio, S. Meller, G. Chamilos, R. Sebasigari, V. Riccieri, R. Bassett, H. Amuro, S. Fukuhara, T. Ito, Y.‐J. Liu, M. Gilliet, Sci. Transl. Med. 2011, 3, 73ra19.
dc.identifier.citedreferenceE. Villanueva, S. Yalavarthi, C. C. Berthier, J. B. Hodgin, R. Khandpur, A. M. Lin, C. J. Rubin, W. Zhao, S. H. Olsen, M. Klinker, D. Shealy, M. F. Denny, J. Plumas, L. Chaperot, M. Kretzler, A. T. Bruce, M. J. Kaplan, J. Immunol. 2011, 187, 538.
dc.identifier.citedreferenceS. Yousefi, J. A. Gold, N. Andina, J. J. Lee, A. M. Kelly, E. Kozlowski, I. Schmid, A. Straumann, J. Reichenbach, G. J. Gleich, H.‐U. Simon, Nat. Med. 2008, 14, 949.
dc.identifier.citedreferenceS. Yousefi, C. Mihalache, E. Kozlowski, I. Schmid, H. U. Simon, Cell Death Differ. 2009, 16, 1438.
dc.identifier.citedreferenceJ. Xu, X. Zhang, M. Monestier, N. L. Esmon, C. T. Esmon, J. Immunol. 2011, 187, 2626.
dc.identifier.citedreferenceC. F. Urban, D. Ermert, M. Schmid, U. Abu‐Abed, C. Goosmann, W. Nacken, V. Brinkmann, P. R. Jungblut, A. Zychlinsky, PLoS Pathog. 2009, 5, e1000639.
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
adhm201900926.pdf
Size:
1.465MB
Format:
PDF
View/Open
Name:
adhm201900926_am.pdf
Size:
1.276MB
Format:
PDF
View/Open
Name:
adhm201900926-sup-0001-SuppMat.pdf
Size:
2.077MB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.