Culturomic and quantitative real- time- polymerase chain reaction analyses for early contamination of abutments with different surfaces: A randomized clinical trial
Canullo, Luigi; Masucci, Luca; Quaranta, Gianluca; Patini, Romeo; Caponio, Vito Carlo Alberto; Pesce, Paolo; Ravidà, Andrea; Penarrocha‐oltra, David; Penarrocha‐diago, Miguel
2021-08
Citation
Canullo, Luigi; Masucci, Luca; Quaranta, Gianluca; Patini, Romeo; Caponio, Vito Carlo Alberto; Pesce, Paolo; Ravidà, Andrea ; Penarrocha‐oltra, David ; Penarrocha‐diago, Miguel (2021). "Culturomic and quantitative real- time- polymerase chain reaction analyses for early contamination of abutments with different surfaces: A randomized clinical trial." Clinical Implant Dentistry and Related Research 23(4): 568-578.
Abstract
BackgroundRough and/or plasma- activated abutments seem to be able to increase soft tissue adhesion and stability; however, limited evidence is available about bacterial contamination differences.PurposeThe aim was to investigate the oral microbiota on four dental abutments with different surfaces by quantitative real- time polymerase chain reaction (qRT- PCR) and culturomic approach.MethodsForty patients needing a single implant rehabilitation were involved in the study. Forty healing abutments, especially designed for the study, were divided into four groups according to the surface topography (1. machined [MAC], 2. machined plasma of argon treated [plasmaMAC], 3. ultrathin threaded microsurface [UTM], 4. UTM plasma of argon treated [Plasma UTM]). Random assignment was performed according to predefined randomization tables. All patients underwent surgical intervention for implant and contextual healing abutment positioning. After 2 months of healing, a sterile cotton swab was used for microbiological sampling for culturomics, while sterile paper points inserted into the sulcus were used for qRT- PCR.ResultsAt the end of the study, 36 patients completed all procedures and a total of 36 abutments (9 per group) were analyzed. qRT- PCR retrieved data for 23 bacterial species whereas culturomics revealed the presence of 74 different bacteria, most of them not routinely included into oral cavity microbiological kits of analysis or never found before in the oral microenvironment. No statistically significant differences emerged analyzing the four different surfaces (p =- 0.053). On the contrary, higher total and specific bacterial counts were detected in the plasma- treated surfaces compared to the untreated ones (p =- 0.021).ConclusionsAbutments with different topographies and surface treatments resulted contaminated by similar oral bacterial flora. Abutments with moderately rough surface were not associated with a greater bacterial adhesion compared to machined ones. Conversely, more bacteria were found around plasma- treated abutments. Furthermore, data reported suggested to include new species not previously sought in the routine analyses of the oral bacterial microflora.Publisher
John Wiley & Sons, Inc.
ISSN
1523-0899 1708-8208
Other DOIs
Types
Article
Metadata
Show full item recordCollections
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.