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Oral biofilm dysbiosis during experimental periodontitis

dc.contributor.authorRibeiro, Apoena Aguiar
dc.contributor.authorJiao, Yizu
dc.contributor.authorGirnary, Mustafa
dc.contributor.authorAlves, Tomaz
dc.contributor.authorChen, Liang
dc.contributor.authorFarrell, Anna
dc.contributor.authorWu, Di
dc.contributor.authorTeles, Flavia
dc.contributor.authorInohara, Naohiro
dc.contributor.authorSwanson, Karen V.
dc.contributor.authorMarchesan, Julie T.
dc.date.accessioned2022-12-05T16:41:39Z
dc.date.available2024-01-05 11:41:37en
dc.date.available2022-12-05T16:41:39Z
dc.date.issued2022-12
dc.identifier.citationRibeiro, Apoena Aguiar; Jiao, Yizu; Girnary, Mustafa; Alves, Tomaz; Chen, Liang; Farrell, Anna; Wu, Di; Teles, Flavia; Inohara, Naohiro; Swanson, Karen V.; Marchesan, Julie T. (2022). "Oral biofilm dysbiosis during experimental periodontitis." Molecular Oral Microbiology 37(6): 256-265.
dc.identifier.issn2041-1006
dc.identifier.issn2041-1014
dc.identifier.urihttps://hdl.handle.net/2027.42/175239
dc.description.abstractObjectivesWe have previously characterized the main osteoimmunological events that occur during ligature periodontitis. This study aims to determine the polymicrobial community shifts that occur during disease development.MethodsPeriodontitis was induced in C57BL/6 mice using the ligature-induced periodontitis model. Healthy oral mucosa swabs and ligatures were collected every 3 days from 0 to 18 days post-ligature placement. Biofilm samples were evaluated by 16SrRNA gene sequencing (Illumina MiSeq) and QIIME. Time-course changes were determined by relative abundance, diversity, and rank analyses (PERMANOVA, Bonferroni-adjusted).ResultsMicrobial differences between health and periodontal inflammation were observed at all phylogenic levels. An evident microbial community shift occurred in 25 genera during the advancement of “gingivitis” (3–6 days) to periodontitis (9–18 days). From day 0 to 18, dramatic changes were identified in Streptococcus levels, with an overall decrease (54.04%–0.02%) as well an overall increase of Enterococcus and Lactobacillus (23.7%–73.1% and 10.1%–70.2%, respectively). Alpha-diversity decreased to its lowest at 3 days, followed by an increase in diversity as disease advancement. Beta-diversity increased after ligature placement, indicating that bone loss develops in response to a greater microbial variability (p = 0.001). Levels of facultative and strict anaerobic bacteria augmented over the course of disease progression, with a total of eight species significantly different during the 18-day period.ConclusionThe data supports that murine gingival inflammation and alveolar bone loss develop in response to microbiome shifts. Bacterial diversity increased during progression to bone loss. These findings further support the utilization of the periodontitis ligature model for microbial shift analysis under different experimental conditions.The shift in microbiome community during periodontitis development in a murine model. Gingival inflammation and bone loss in murine periodontitis develops in response to microbiome shifts, marked by a decrease in alpha diversity in the initial stages, followed by an increase in alpha and beta diversities as disease progresses.
dc.publisherWiley Periodicals, Inc.
dc.subject.otherbiofilm
dc.subject.otherexperimental periodontitis
dc.subject.otherdysbiosis
dc.titleOral biofilm dysbiosis during experimental periodontitis
dc.typeArticle
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biology
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/175239/1/omi12389-sup-0001-tableS1.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/175239/2/omi12389.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/175239/3/omi12389_am.pdf
dc.identifier.doi10.1111/omi.12389
dc.identifier.sourceMolecular Oral Microbiology
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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