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Modeling Asthma in Mice Using Rhinovirus Infection

dc.contributor.authorBentley, JK
dc.coverage.spatialUnited States
dc.date.accessioned2023-02-01T17:46:30Z
dc.date.available2023-02-01T17:46:30Z
dc.date.issued2022-01-01
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pubmed/35771462
dc.identifier.urihttps://hdl.handle.net/2027.42/175723en
dc.description.abstractRhinovirus (RV) infection is linked to early life wheezing and exacerbation of adult asthma. RV infection can be modeled in adult and neonatal mice. This chapter outlines methods for the production of standardized human rhinovirus A1B and mouse infection. The chapter also describes methods to couple infections with allergen (ovalbumin and house dust mite) administrations. The production of the virus involves its amplification, purification, and concentration. In order to standardize the concentrated RV stock, a plaque assay on HeLa cells is outlined as a method of calibrating infectivity. Once the number of plaque-forming units is determined, the standardized virus is used for mouse infection.
dc.format.mediumPrint
dc.languageeng
dc.publisherSpringer Nature
dc.subjectAirways
dc.subjectAsthma
dc.subjectInflammation
dc.subjectMouse
dc.subjectRhinovirus
dc.subjectAnimals
dc.subjectAsthma
dc.subjectEnterovirus Infections
dc.subjectHeLa Cells
dc.subjectHumans
dc.subjectMice
dc.subjectPicornaviridae Infections
dc.subjectPyroglyphidae
dc.subjectRhinovirus
dc.titleModeling Asthma in Mice Using Rhinovirus Infection
dc.typeArticle
dc.identifier.pmid35771462
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/175723/2/Bentley2022_Protocol_ModelingAsthmaInMiceUsingRhino.pdf
dc.identifier.doi10.1007/978-1-0716-2364-0_3
dc.identifier.doihttps://dx.doi.org/10.7302/6937
dc.identifier.sourceMethods Mol Biol
dc.description.versionPublished version
dc.date.updated2023-02-01T17:46:28Z
dc.identifier.orcid0000-0001-8865-7979
dc.identifier.volume2506
dc.identifier.startpage43
dc.identifier.endpage56
dc.identifier.name-orcidBentley, JK; 0000-0001-8865-7979
dc.working.doi10.7302/6937en
dc.owningcollnamePediatrics and Communicable Diseases, Department of


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