Urea amidolyase of Candida utilis : Characterization of the urea cleavage reactions
dc.contributor.author | Castric, Peter A. | en_US |
dc.contributor.author | Levenberg, Bruce | en_US |
dc.date.accessioned | 2006-04-07T16:27:23Z | |
dc.date.available | 2006-04-07T16:27:23Z | |
dc.date.issued | 1976-07-08 | en_US |
dc.identifier.citation | Castric, Peter A., Levenberg, Bruce (1976/07/08)."Urea amidolyase of Candida utilis : Characterization of the urea cleavage reactions." Biochimica et Biophysica Acta (BBA) - Enzymology 438(2): 574-583. <http://hdl.handle.net/2027.42/21726> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B73GH-47GH65P-2B/2/193d5cb4bc39ca44a0b22dc092e1b844 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/21726 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8111&dopt=citation | en_US |
dc.description.abstract | Evidence is presented that the enzymes catalyzing the three reactions involved in urea cleavage in Candida utilis, biotin carboxylation, urea carboxylation, and allophanate hydrolysis occur as a complex of enzymes. The allophanatehydrolyzing activity could not be separated from the urea-cleaving activity using common methods of protein purification. Further, urea cleavage and allophanate hydrolysis activities are induced coordinately in cells grown on various nitrogen sources.The reactions involved in urea cleavage can be distinguished from one another on the basis of their sensitivities to (a) heat, (b) pH, and (c) chemical inhibitors. Evidence is presented for the product of the first reaction in urea cleavage, biotin carboxylation. Production of carboxylated enzyme is ATP dependent and avidin sensitive. Carboxylated enzyme is not observed in the presence of 1 mM urea. | en_US |
dc.format.extent | 654418 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Urea amidolyase of Candida utilis : Characterization of the urea cleavage reactions | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Mich. 48104, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Biological Sciences, Duquesne University, Pittsburgh, Pa. 15219, U.S.A. | en_US |
dc.identifier.pmid | 8111 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/21726/1/0000118.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0005-2744(76)90273-4 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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