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Dye titrations of covalently closed supercoiled DNA analyzed by agarose gel electrophoresis
dc.contributor.authorDeLeys, Robert J.en_US
dc.contributor.authorJackson, David A.en_US
dc.date.accessioned2006-04-07T16:29:52Z
dc.date.available2006-04-07T16:29:52Z
dc.date.issued1976-03-22en_US
dc.identifier.citationDeLeys, Robert J., Jackson, David A. (1976/03/22)."Dye titrations of covalently closed supercoiled DNA analyzed by agarose gel electrophoresis." Biochemical and Biophysical Research Communications 69(2): 446-454. <http://hdl.handle.net/2027.42/21806>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WBK-4DYM9YK-T0/2/d16bf9ba398fb4a7c132a1b3438e5be9en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/21806
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=178312&dopt=citationen_US
dc.description.abstractWe have developed a rapid electrophoretic technique for performing ethidium bromide dye titrations in cylindrical 0.7% agarose gels. The technique was used to analyze the extent of supercoiling in circular covalently closed SV40, Co1E1, and pSC101 DNA. We have estimated the superhelical densities of SV40, Co1E1, and pSC101 DNA to be -0.050, -0.078, and -0.085 respectively. The results obtained for native SV40 DNA correlate well with previously published values for the superhelical density of this DNA when these values are corrected to reflect a 26[deg] duplex unwinding angle for ethidium bromide. Ethidium bromide concentrations sufficient to partially relax a supercoiled DNA allow the DNA to be resolved into a series of discrete bands in agarose gels. The distribution of bands represents a natural heterogeneity in the superhelical densities of the DNA molecules in the population.en_US
dc.format.extent2787756 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleDye titrations of covalently closed supercoiled DNA analyzed by agarose gel electrophoresisen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Microbiology University of Michigan Medical School, Ann Arbor, Michigan, U.S.A. 48109en_US
dc.contributor.affiliationumDepartment of Microbiology University of Michigan Medical School, Ann Arbor, Michigan, U.S.A. 48109en_US
dc.identifier.pmid178312en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/21806/1/0000206.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0006-291X(76)90542-8en_US
dc.identifier.sourceBiochemical and Biophysical Research Communicationsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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