The effect of proteoglycans on the formation of fibrils from collagen solutions
dc.contributor.author | Oegema, Jr. , Theodore R. | en_US |
dc.contributor.author | Laidlaw, Jana | en_US |
dc.contributor.author | Hascall, Vincent C. | en_US |
dc.contributor.author | Dziewiatkowski, Dominic D. | en_US |
dc.date.accessioned | 2006-04-07T16:41:47Z | |
dc.date.available | 2006-04-07T16:41:47Z | |
dc.date.issued | 1975 | en_US |
dc.identifier.citation | Oegema, Jr., Theodore R., Laidlaw, Jana, Hascall, Vincent C., Dziewiatkowski, Dominic D. (1975)."The effect of proteoglycans on the formation of fibrils from collagen solutions." Archives of Biochemistry and Biophysics 170(): 698-709. <http://hdl.handle.net/2027.42/22193> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WB5-4DW2FWY-XJ/2/b5a8d47ccaa2f71d2ef90514f54e2082 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22193 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=127547&dopt=citation | en_US |
dc.description.abstract | The precipitation of collagen fibrils from solutions at 37 [deg]C and approximately physiological pH and ionic strength is retarded markedly in the presence of small amounts of proteoglycan monomer (PGS), proteoglycan aggregate (PGC), reduced and alkylated PGS, or cyanogen bromide-cleavage products of PGS. The polysaccharide-peptide fragments produced from PGS with papain, trypsin, cathepsin D, or the protein core obtained by the digestion of PGS with chondroitinase ABC are ineffective in this regard. In the presence of the materials which affected the rate of precipitation of the collagen fibrils, the specific absorbance, [Delta]Asp, of the collagen gels was directly related to specific retardation, Rsp, when the ratio of proteoglycan to collagen was less than 25 [mu]g/mg, suggesting that the size and/or organization of the fibrils in the gels was dependent on the presence of proteoglycans. PGS binds to collagen if it is present in the solution before the collagen fibrils form and at a maximum of about 1 molecule of PGS for every 25-30 molecules of collagen. Although the protein core of PGS does not retard fibrillogenesis, it does bind to collagen and does so even in the presence of PGS. The data support the thesis that the organization of collagen fibrils in tissues may be related to amounts and kinds of proteoglycans in the tissues. | en_US |
dc.format.extent | 1092848 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | The effect of proteoglycans on the formation of fibrils from collagen solutions | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | The Departments of Oral Biology and Biological Chemistry andd The Dental Research Institute, The University of Michigan, Ann Arbor, Michigan 48104, USA | en_US |
dc.contributor.affiliationum | The Departments of Oral Biology and Biological Chemistry andd The Dental Research Institute, The University of Michigan, Ann Arbor, Michigan 48104, USA | en_US |
dc.contributor.affiliationum | The Departments of Oral Biology and Biological Chemistry andd The Dental Research Institute, The University of Michigan, Ann Arbor, Michigan 48104, USA | en_US |
dc.contributor.affiliationother | no department found | en_US |
dc.identifier.pmid | 127547 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22193/1/0000624.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-9861(75)90167-8 | en_US |
dc.identifier.source | Archives of Biochemistry and Biophysics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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