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Cytochrome P-450 purified to apparent homogeneity from phenobarbital-induced rabbit liver microsomes: Catalytic activity and other properties

dc.contributor.authorvan der Hoeven, Theodore A.en_US
dc.contributor.authorHaugen, David A.en_US
dc.contributor.authorCoon, Minor J.en_US
dc.date.accessioned2006-04-07T16:44:08Z
dc.date.available2006-04-07T16:44:08Z
dc.date.issued1974-09-23en_US
dc.identifier.citationvan der Hoeven, Theodore A., Haugen, David A., Coon, Minor J. (1974/09/23)."Cytochrome P-450 purified to apparent homogeneity from phenobarbital-induced rabbit liver microsomes: Catalytic activity and other properties." Biochemical and Biophysical Research Communications 60(2): 569-575. <http://hdl.handle.net/2027.42/22272>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WBK-4DNB473-11N/2/02b332bf89c441fd2291bcb1ad109140en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/22272
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4418358&dopt=citationen_US
dc.description.abstractCytochrome P-450 was purified to a content of over 17 nmoles per mg of protein from liver microsomes of phenobarbital-treated rabbits by fractionation with polyethylene glycol 6000, DEAE-cellulose column chromatography, and hydroxylapatite column chromatography in the presence of Renex 690, a nonionic detergent. The purified preparation exhibited a single polypeptide band (molecular weight, 49,000 daltons) when submitted to SDS-polyacrylamide gel electrophoresis. Cytochromes P-420 and 5 and NADPH-cytochrome reductase were absent. The reconstituted system containing purified cytochrome P-450, reductase, and phosphatidylcholine catalyzed the hydroxylation of benzphetamine, cyclohexane, aniline, and laurate.en_US
dc.format.extent594597 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleCytochrome P-450 purified to apparent homogeneity from phenobarbital-induced rabbit liver microsomes: Catalytic activity and other propertiesen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry Medical School, The University of Michigan, Ann Arbor, Michigan 48104, USAen_US
dc.contributor.affiliationumDepartment of Biological Chemistry Medical School, The University of Michigan, Ann Arbor, Michigan 48104, USAen_US
dc.contributor.affiliationumDepartment of Biological Chemistry Medical School, The University of Michigan, Ann Arbor, Michigan 48104, USAen_US
dc.identifier.pmid4418358en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/22272/1/0000711.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0006-291X(74)90278-2en_US
dc.identifier.sourceBiochemical and Biophysical Research Communicationsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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