Isolation of plasma membranes from bovine corpus luteum possessing adenylate cyclase, 125I-hCG binding and Na---K-ATPase activities

Abstract

Plasma membranes from bovine corpora lutea have been purified by sucrose density gradient centrifugation. The purified membranes, in addition to binding 125I-hCG, also possess hCG-stimulated adenylate cyclase and Na---K-ATPase. The relative purification of 125I-hCG binding, adenylate cyclase and Na---K-ATPase on the basis of the specific activities in the whole homogenate were 7.8, 6.4 and 2.6, respectively. The presence of both the hormone sensitive adenylate cyclase and 125I-hCG binding activities suggest that these plasma membranes might possess the `receptor' for gonadotropin.