Lipid extraction of tissues with a low-toxicity solvent
dc.contributor.author | Hara, Atsushi | en_US |
dc.contributor.author | Radin, Norman S. | en_US |
dc.date.accessioned | 2006-04-07T16:58:50Z | |
dc.date.available | 2006-04-07T16:58:50Z | |
dc.date.issued | 1978-10-01 | en_US |
dc.identifier.citation | Hara, Atsushi, Radin, Norman S. (1978/10/01)."Lipid extraction of tissues with a low-toxicity solvent." Analytical Biochemistry 90(1): 420-426. <http://hdl.handle.net/2027.42/22520> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6W9V-4DYM9HN-6M/2/f94b22510b8d82da68c4a2441fdc007e | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22520 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=727482&dopt=citation | en_US |
dc.description.abstract | An improved method for extracting the lipids from tissues consists of the use of hexane:isopropanol, followed by a wash of the extract with aqueous sodium sulfate to remove nonlipid contaminants. This method has a number of advantages over the common usage of chloroform:methanol. The solvents are somewhat less toxic, interference in processing by proteolipid protein contamination is avoided, the two phase separate rapidly during the washing step, the solvent density is low enough to permit centrifugation of the homogenate as an alternative to filtration, the solvents are cheaper, and the washed extract can be applied to a chromatographic column with continuous monitoring of the elution in the far ultraviolet region. The new extraction method is inefficient for the extraction of gangliosides. | en_US |
dc.format.extent | 454345 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Lipid extraction of tissues with a low-toxicity solvent | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry University of Michigan, Ann Arbor, Michigan 48109, USA: Mental Health Research Institute University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry University of Michigan, Ann Arbor, Michigan 48109, USA: Mental Health Research Institute University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 727482 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22520/1/0000064.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-2697(78)90046-5 | en_US |
dc.identifier.source | Analytical Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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