Aliphatic hydroxylation by highly purified liver microsomal cytochrome P-450. Evidence for a carbon radical intermediate
dc.contributor.author | Groves, John T. | en_US |
dc.contributor.author | McClusky, Gary A. | en_US |
dc.contributor.author | White, Ronald E. | en_US |
dc.contributor.author | Coon, Minor J. | en_US |
dc.date.accessioned | 2006-04-07T17:02:14Z | |
dc.date.available | 2006-04-07T17:02:14Z | |
dc.date.issued | 1978-03-15 | en_US |
dc.identifier.citation | Groves, John T., McClusky, Gary A., White, Ronald E., Coon, Minor J. (1978/03/15)."Aliphatic hydroxylation by highly purified liver microsomal cytochrome P-450. Evidence for a carbon radical intermediate." Biochemical and Biophysical Research Communications 81(1): 154-160. <http://hdl.handle.net/2027.42/22630> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4DXRY44-2SB/2/2597bed4a3e581bd2bf23e0b4014598a | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22630 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=656092&dopt=citation | en_US |
dc.description.abstract | The oxidation of norbornane by a reconstituted liver cytochrome P-450 system affords - and -2-norborneol in a ratio of 3.4:1. The ratio of these products was found to be 0.76:1 when -2,3,5,6-tetradueteronorbornane was oxidized. Analysis of the mass spectra of the products from the deuterated hydrocarbon showed that 25% of the -norborneol contained four deuterium atoms whereas 9% of the -norborneol contained three deuterium atoms. These results, which indicate a very large isotope effect (kH/kD = 11.5+/-1) and a significant amount of epimerization for the hydroxylation of norbornane by cytochrome P-450, suggest an initial hydrogen abstraction to give a carbon radical intermediate. | en_US |
dc.format.extent | 399881 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Aliphatic hydroxylation by highly purified liver microsomal cytochrome P-450. Evidence for a carbon radical intermediate | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemistry The University of Michigan, Ann Arbor, Michigan 48109, USA; Department of Biological Chemistry The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry The University of Michigan, Ann Arbor, Michigan 48109, USA; Department of Biological Chemistry The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry The University of Michigan, Ann Arbor, Michigan 48109, USA; Department of Biological Chemistry The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry The University of Michigan, Ann Arbor, Michigan 48109, USA; Department of Biological Chemistry The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 656092 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22630/1/0000180.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-291X(78)91643-1 | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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