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Plasma ADP levels: Direct determination with luciferase luminescence using a biometer

dc.contributor.authorJabs, Clarence M.en_US
dc.contributor.authorFerrell, William J.en_US
dc.contributor.authorRobb, Herbert J.en_US
dc.date.accessioned2006-04-07T17:05:05Z
dc.date.available2006-04-07T17:05:05Z
dc.date.issued1978en_US
dc.identifier.citationJabs, Clarence M., Ferrell, William J., Robb, Herbert J. (1978)."Plasma ADP levels: Direct determination with luciferase luminescence using a biometer." Clinical Biochemistry 11(5): 190-193. <http://hdl.handle.net/2027.42/22719>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6TDD-4GXVY2S-1/2/c1c33cb31688a69f2bfd3cd5cfdaeba6en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/22719
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=729160&dopt=citationen_US
dc.description.abstractA method is described for the determination of low plasma levels of adenosine-5'-diphosphate (ADP) using a Dupont Biometer to measure luminescence produced by the luciferin-luciferase reaction. Endogenous ATP is removed by incubation with luciferase. The remaining ADP is then quantitated, following its conversion to ATP, after incubation with creatine phosphate and creatine kinase. The mean coefficient of variation for 0.02 and 2.2 [mu]mol/liter ADP standards were 2.1 and 1.8% respectively. The method has been applied to human and rabbit plasma. Human plasma ADP concentrations were found to be 0.13 [plus-or-minus sign] 0.025 (10) [mu]mol/liter and rabbit plasma concentration were 0.07 [plus-or-minus sign] 0.05 (5) [mu]mol/liter. Several other possible applications of the method are discussed.en_US
dc.format.extent379595 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titlePlasma ADP levels: Direct determination with luciferase luminescence using a biometeren_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPathologyen_US
dc.subject.hlbsecondlevelDentistryen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pathology and Biological Chemistry, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationotherWilliam Beaumont Hospital, Department of Surgery, Royal Oak, Michigan 48072, USAen_US
dc.contributor.affiliationotherWilliam Beaumont Hospital, Department of Surgery, Royal Oak, Michigan 48072, USAen_US
dc.identifier.pmid729160en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/22719/1/0000274.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/S0009-9120(78)80027-7en_US
dc.identifier.sourceClinical Biochemistryen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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