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A revised fluorometric assay for Gaucher's disease using conduritol-[beta]-epoxide with liver as the source of [beta]-glucosidase

dc.contributor.authorDaniels, Lydia B.en_US
dc.contributor.authorGlew, Robert H.en_US
dc.contributor.authorRadin, Norman S.en_US
dc.contributor.authorVunnam, Ranga R.en_US
dc.date.accessioned2006-04-07T17:21:32Z
dc.date.available2006-04-07T17:21:32Z
dc.date.issued1980-09-25en_US
dc.identifier.citationDaniels, Lydia B., Glew, Robert H., Radin, Norman S., Vunnam, Ranga R. (1980/09/25)."A revised fluorometric assay for Gaucher's disease using conduritol-[beta]-epoxide with liver as the source of [beta]-glucosidase." Clinica Chimica Acta 106(2): 155-163. <http://hdl.handle.net/2027.42/23143>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T57-47RB2KC-264/2/c8633610ce2c7b573f4dbddf170e0b18en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/23143
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6773704&dopt=citationen_US
dc.description.abstractTo date, enzymatic diagnosis of Gaucher's disease via a fluorometric assay procedure which utilizes 4-methylumbelliferyl-[beta]--glucopyranoside as a substrate has not been possible when liver serves as the source of enzyme since currently employed fluorometric procedures cannot adequately differentiate between a broad-specificity [beta]-glucosidase and lysosomal glucocerebrosidase activities in crude extracts of liver. Incorporation of conduritol-[beta]-epoxide into the incubation medium for the fluorometric assay allows one to selectively measure the glucocerebrosidase activity present in a given liver extract. In five cases of Gaucher's disease this revised fluorometric procedure proved as effective as the assay procedure which utilizes authentic, radiolabeled glucocerebroside as the substrate in demonstrating a deficiency of glucocerebrosidase activity in liver.en_US
dc.format.extent790204 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleA revised fluorometric assay for Gaucher's disease using conduritol-[beta]-epoxide with liver as the source of [beta]-glucosidaseen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPathologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Psychiatry, Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, U.S.A.en_US
dc.contributor.affiliationumDepartment of Psychiatry, Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, U.S.A.en_US
dc.contributor.affiliationotherDepartment of Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, U.S.A.en_US
dc.contributor.affiliationotherDepartment of Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, U.S.A.en_US
dc.identifier.pmid6773704en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/23143/1/0000067.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0009-8981(80)90168-0en_US
dc.identifier.sourceClinica Chimica Actaen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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