View Item 

Show simple item record

Purification of bovine liver microsomal NADH-cytochrome reductase using affinity chromatography

dc.contributor.authorSchafer, Dorothy A.en_US
dc.contributor.authorHultquist, Donald E.en_US
dc.date.accessioned2006-04-07T17:23:20Z
dc.date.available2006-04-07T17:23:20Z
dc.date.issued1980-07-16en_US
dc.identifier.citationSchafer, Dorothy A., Hultquist, Donald E. (1980/07/16)."Purification of bovine liver microsomal NADH-cytochrome reductase using affinity chromatography." Biochemical and Biophysical Research Communications 95(1): 381-387. <http://hdl.handle.net/2027.42/23200>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WBK-4DN95M1-10S/2/a9e05830baa2ffa5e7ab35ce4b1a806cen_US
dc.identifier.urihttps://hdl.handle.net/2027.42/23200
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7417264&dopt=citationen_US
dc.description.abstractMicrosomal NADH-cytochrome reductase has been purified from bovine liver by an improved procedure which employs affinity chromatography on ADP-agarose in combination with anion exchange chromatography. The reductase was extracted from a 105,000 x microsomal pellet with Triton X-100. The overall purification from isolated microsomes was 98-fold and the yield was 10%. The preparation was nearly homogeneous on SDS-PAGE. This procedure requires less time and effort than previously described procedures. Partially purified cytochrome is also obtained.en_US
dc.format.extent586621 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titlePurification of bovine liver microsomal NADH-cytochrome reductase using affinity chromatographyen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry The University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Biological Chemistry The University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.identifier.pmid7417264en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/23200/1/0000127.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0006-291X(80)90749-4en_US
dc.identifier.sourceBiochemical and Biophysical Research Communicationsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
0000127.pdf
Size:
572.8KB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.