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The influence of mitogen costimulation on the human lymphocyte blastogenic response to Actinomyces viscosus ultrasonicates

dc.contributor.authorLopatin, Dennis E.en_US
dc.contributor.authorPeebles, Frances L.en_US
dc.contributor.authorHorner, Irene S.en_US
dc.date.accessioned2006-04-07T17:24:49Z
dc.date.available2006-04-07T17:24:49Z
dc.date.issued1980-05en_US
dc.identifier.citationLopatin, Dennis E., Peebles, Frances L., Horner, Irene S. (1980/05)."The influence of mitogen costimulation on the human lymphocyte blastogenic response to Actinomyces viscosus ultrasonicates." Clinical Immunology and Immunopathology 16(1): 75-83. <http://hdl.handle.net/2027.42/23248>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WCK-4BJW1SS-MK/2/cd0caa53b786db8c8e7489552ae55d72en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/23248
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6966555&dopt=citationen_US
dc.description.abstractHuman peripheral blood lymphocytes were cultured for 48 hr in the presence of an ultrasonicate supernatant fraction of Actinomyces viscosus (AV-SUP). After washing to remove AV-SUP, the lymphocytes were cultured in the presence of concanavalin A or pokeweed mitogen for an additional 72 hr. Measurement of [3H]thymidine incorporation into DNA indicated that AV-SUP preculture resulted in an amplified or elevated response to mitogen stimulation compared to mock precultured cells and unstimulated controls. Addition of fresh cells prior to mitogen activation resulted in an additional incremental increase over controls. The modulated cell appeared to reside in the AV-SUP precultured population, since treatment of these cells with mitomycin C prior to coculture with fresh cells totally abrogated the effect. However, mitomycin C treatment of the fresh population had no effect on mitogen-induced amplification. Results of these experiments suggest that mitogen stimulation activates a helper function which allows the precultured cells to respond to AV-SUP stimulation.en_US
dc.format.extent672398 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleThe influence of mitogen costimulation on the human lymphocyte blastogenic response to Actinomyces viscosus ultrasonicatesen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbsecondlevelInternal Medicine and Specialtiesen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Oral Biology, University of Michigan, Ann Arbor, Michigan 48109, USA: Department of Microbiology-Immunology, University of Michigan, Ann Arbor, Michigan 48109, USA: Dental Research Institute, University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Oral Biology, University of Michigan, Ann Arbor, Michigan 48109, USA: Department of Microbiology-Immunology, University of Michigan, Ann Arbor, Michigan 48109, USA: Dental Research Institute, University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Oral Biology, University of Michigan, Ann Arbor, Michigan 48109, USA: Department of Microbiology-Immunology, University of Michigan, Ann Arbor, Michigan 48109, USA: Dental Research Institute, University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.identifier.pmid6966555en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/23248/1/0000181.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0090-1229(80)90168-3en_US
dc.identifier.sourceClinical Immunology and Immunopathologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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