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Paired-ion high-performance liquid chromatographic assay for plasma choline

dc.contributor.authorBuchanan, Douglas N.en_US
dc.contributor.authorFucek, Franz R.en_US
dc.contributor.authorDomino, Edward F.en_US
dc.date.accessioned2006-04-07T17:26:13Z
dc.date.available2006-04-07T17:26:13Z
dc.date.issued1980-03-14en_US
dc.identifier.citationDouglas N., Buchanan, Franz R Fucek, , Edward F Domino, (1980/03/14)."Paired-ion high-performance liquid chromatographic assay for plasma choline." Journal of Chromatography B: Biomedical Sciences and Applications 181(3-4): 329-335. <http://hdl.handle.net/2027.42/23290>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6TG9-44BNNBY-2X/2/3362ce1a9581e145d6a05a1de6d1c4e0en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/23290
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7391149&dopt=citationen_US
dc.description.abstractCholine was isolated from deproteinized plasma by cation-exchange chromatography. Isolated choline was directly converted to the 3,5-dinitrobenzoate derivative and was analyzed by paired-ion high-performance liquid chromatography with UV detection at 254 nm. An internal standard, 3-hydroxy-N,N,N-trimethylpropanaminium iodide was used for quantitation of plasma choline.Linearity was achieved from 1-500 nmole/ml with a reproducibility of +/- 6%. Plasma choline concentrations below 1 nmole/ml could not be accurately measured while plasma choline concentrations in the [mu]mole/ml range deviated from linearity.en_US
dc.format.extent618218 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titlePaired-ion high-performance liquid chromatographic assay for plasma cholineen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumThe University of Michigan, Ann Arbor, Mich. 48109 U.S.A.en_US
dc.contributor.affiliationumThe University of Michigan, Ann Arbor, Mich. 48109 U.S.A.en_US
dc.contributor.affiliationotherDepartment of Pharmacology, Lafayette Clinic, 951 E. Lafayette, Detroit, Mich. 48207 U.S.A.en_US
dc.identifier.pmid7391149en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/23290/1/0000227.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/S0378-4347(00)81135-4en_US
dc.identifier.sourceJournal of Chromatography B: Biomedical Sciences and Applicationsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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