Analysis of sperm antigens by sodium dodecyl sulfate gel/protein blot radioimmunobinding method
dc.contributor.author | Lee, Chi-Yu Gregory | en_US |
dc.contributor.author | Huang, Yuan-Shen | en_US |
dc.contributor.author | Hu, Ping-Chuan | en_US |
dc.contributor.author | Gomel, Victor | en_US |
dc.contributor.author | Menge, Alan C. | en_US |
dc.date.accessioned | 2006-04-07T17:51:15Z | |
dc.date.available | 2006-04-07T17:51:15Z | |
dc.date.issued | 1982-06 | en_US |
dc.identifier.citation | Lee, Chi-Yu Gregory, Huang, Yuan-Shen, Hu, Ping-Chuan, Gomel, Victor, Menge, Alan C. (1982/06)."Analysis of sperm antigens by sodium dodecyl sulfate gel/protein blot radioimmunobinding method." Analytical Biochemistry 123(1): 14-22. <http://hdl.handle.net/2027.42/23957> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6W9V-4DX4BB7-127/2/a07c8f559629edd9b890695537031587 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23957 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7114466&dopt=citation | en_US |
dc.description.abstract | A radioimmunobinding method based on the blotting of renatured proteins from sodium dodecyl sulfate gels on to nitrocellulose filter papers was developed to analyze the sperm antigens that elicit serum anti-sperm antibodies. In rabbits, serum anti-sperm antibodies were raised by immunization with homologous epididymal spermatozoa mixed with complete Freund's adjuvant. The raised antisera from either male or female rabbits were shown to react with three major sperm protein bands on sodium dodecyl sulfate gels with the corresponding molecular weights of about 70,000 +/- 5000, 14,000, and 13,000, respectively. In humans, the monoclonal antibodies against human sperm were raised by a hybridoma technique. Out of six independent hybrid cell lines that were generated, three of them were shown to secrete immunoglobulins that react with the same two protein bands on sodium dodecyl sulfate gels, which have the approximate molecular weight of 10,000. The same procedure was also used to analyze human serum samples that were shown to contain anti-sperm antibodies by the known techniques. Unique sperm antigens that elicit anti-sperm antibodies in humans were identified and correlated. The results of this study suggest that sodium dodecyl sulfate gel/protein blot radioimmunobinding method may be a sensitive and useful tool for the study of sperm antigens that elicit autoimmune responses and their association with human infertility. | en_US |
dc.format.extent | 3816540 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Analysis of sperm antigens by sodium dodecyl sulfate gel/protein blot radioimmunobinding method | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Obstetrics and Gynecology, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationother | Department of Obstetrics and Gynaecology, The University of British Columbia, Vancouver, British Columbia V6T 2B5, Canada | en_US |
dc.contributor.affiliationother | Cancer Research Center, University of North Carolina, Chapel Hill, North Carolina 27514, USA | en_US |
dc.contributor.affiliationother | Cancer Research Center, University of North Carolina, Chapel Hill, North Carolina 27514, USA | en_US |
dc.contributor.affiliationother | Department of Obstetrics and Gynaecology, The University of British Columbia, Vancouver, British Columbia V6T 2B5, Canada | en_US |
dc.identifier.pmid | 7114466 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23957/1/0000206.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-2697(82)90617-0 | en_US |
dc.identifier.source | Analytical Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.