Internucleotide incorporation of arabinosyladenine into herpes simplex virus and mammalian cell DNA
dc.contributor.author | Pelling, Jill C. | en_US |
dc.contributor.author | Drach, John C. | en_US |
dc.contributor.author | Shipman, Charles Jr. | en_US |
dc.date.accessioned | 2006-04-07T18:08:49Z | |
dc.date.available | 2006-04-07T18:08:49Z | |
dc.date.issued | 1981-03 | en_US |
dc.identifier.citation | Pelling, Jill C., Drach, John C., Shipman, JR., Charles (1981/03)."Internucleotide incorporation of arabinosyladenine into herpes simplex virus and mammalian cell DNA." Virology 109(2): 323-335. <http://hdl.handle.net/2027.42/24446> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WXR-4BSF210-9/2/510c36d7f4fe596421544c237fcaedd0 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/24446 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6259814&dopt=citation | en_US |
dc.description.abstract | Viral DNA was isolated from lysates of herpes simplex virus type 1-infected cells which had been incubated in the presence of 0.1, 1.0, or 3.2 [mu]M [3H]ara-A. The DNA was purified by isopycnic centrifugation and was enzymatically digested with micrococcal nuclease and spleen phosphodiesterase. The resulting nucleotides and nucleosides were resolved by means of thin-layer chromatography. Examination of the distribution of radioactivity on the chromatograms revealed that 92-96% of the label was present as nucleotides demonstrating that [3H]ara-A was internally incorporated into viral DNA. Virtually identical results were obtained using DNA isolated from mature virions. Additional analysis of virion DNA using HindIII restriction endonuclease demonstrated that the incorporation of [3H]ara-A into the viral genome was random and did not occur at preferential sites. The amount of ara-A incorporated into viral DNA from infected cells was compared with the amount incorporated into cellular DNA from uninfected cells. Viral DNA isolated from cells maintained in 0.1, 1.0, or 3.2 [mu]M [3H]ara-A, contained 2.3, 9.7, or 27.5 ara-AMP residues/10,000 dAMP residues, respectively, whereas uninfected cellular DNA. contained 4.1, 41.0, or 160 ara-AMP residues/10,000 dAMP residues, respectively. These data establish that (i) ara-A is not an absolute chain terminator of HSV-1 DNA synthesis, and (ii) the amount of ara-A incorporated into viral DNA is less than the amount incorporated into cellular DNA. | en_US |
dc.format.extent | 1593099 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Internucleotide incorporation of arabinosyladenine into herpes simplex virus and mammalian cell DNA | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, School of Medicine, The University of Michigan, Ann Arbor, Michigan 8109, USA | en_US |
dc.contributor.affiliationum | Department of Oral Biology and the Dental Research Institute, School of Dentistry, The University of Michigan, Ann Arbor, Michigan 8109, USA | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, School of Medicine, The University of Michigan, Ann Arbor, Michigan 8109, USA; Department of Oral Biology and the Dental Research Institute, School of Dentistry, The University of Michigan, Ann Arbor, Michigan 8109, USA | en_US |
dc.identifier.pmid | 6259814 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/24446/1/0000720.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0042-6822(81)90503-1 | en_US |
dc.identifier.source | Virology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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