Estimation of acyldihydroxyacetone phosphate and lysophosphatidate in animal tissues
dc.contributor.author | Das, Arun K. | en_US |
dc.contributor.author | Hajra, Amiya K. | en_US |
dc.date.accessioned | 2006-04-07T18:17:37Z | |
dc.date.available | 2006-04-07T18:17:37Z | |
dc.date.issued | 1984-11-14 | en_US |
dc.identifier.citation | Das, Arun K., Hajra, Amiya K. (1984/11/14)."Estimation of acyldihydroxyacetone phosphate and lysophosphatidate in animal tissues." Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 796(2): 178-189. <http://hdl.handle.net/2027.42/24642> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1X-47G34VK-1G/2/fd94ac8a3ab5861e1000f435fe3ad395 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/24642 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6388643&dopt=citation | en_US |
dc.description.abstract | Chemical and enzymatic methods have been developed to measure small quantities (10-8-10-10 mol) of acyldihydroxyacetone phosphate in animal tissues. Lipids extracted from tissue samples with acidic CHCl3/ methanol were subjected to solvent partitioning at two different pH values for partial purification of keto-lipid from other lipids. This lipid was then estimated radiometrically either by chemical reduction with NaB3H4 or by enzymatic reduction with [4B-3H]NADPH using a partially purified acyldihydroxyacetonephosphate reductase (EC 1.1.1.101). Thin-layer chromatography revealed the presence of a number of 3H-labeled lipids in the NaB3H4-reduced product and further purification of the product was necessary to estimate the amount of acyl[2-3H]glycerol 3-phosphate formed. The enzymatic reduction was very specific for acyl/alkyldihydroxy acetone phosphate. The amounts (nmol/g) of these keto-lipids estimated in different tissues by the enzymatic method were 10.06 +/- 0.64 (guinea pig liver), 4.3 +/- 0.15 (rat liver), 2.1 (rat testis), 1.5 (rad kidney) and 1.2 (rat brain). Monoacylglycerol 3-phosphate, i.e., lysophosphatidic acid, which was co-purified with acyldihydroxyacetone phosphate, was found to be present in relatively larger amounts in tissues. The amounts (nmol/g) of this lipid, estimated by enzymatically measuring the amounts of sn-glycerol 3-phosphate released after alkaline methanolysis of the partially purified lipid extracts, were 143 (guinea pig liver), 58 (rat liver), 53 (rat kidney) and 92 (rat brain). Stearic acid (18:0) was found to be the major (65%) fatty acid present in the lysophosphatidate purified from guinea pig liver. | en_US |
dc.format.extent | 1979285 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Estimation of acyldihydroxyacetone phosphate and lysophosphatidate in animal tissues | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory, Mental Health Research Institute and Department of Biological Chemistry, University of Michigan, 1103 E. Huron, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory, Mental Health Research Institute and Department of Biological Chemistry, University of Michigan, 1103 E. Huron, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 6388643 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/24642/1/0000053.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0005-2760(84)90346-1 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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