Prostaglandin biosynthesis does not participate in isoproterenol-induced renin release

Abstract

Rat renal slices were incubated in two different media. One was a normal K. physiological saline solution and the other a high K medium. Renin release was measured every 15 min in the presence and absence of 10-6M isoproterenol and also in the presence and absence of aspirin, 0.8 or 1.6 x 10-5M. In all experiments renin release was linear during the 75 min of incubation. Isoproterenol increased renin release by approximately 100%. This was the case even in the presence of aspirin which significantly inhibited prostaglandin release (PGE2, PGF2 [alpha] and 6-keto-PGF1 [alpha]). Nor was there any reduction in the basal secretory rate by aspirin alone. These data are taken to indicate that aspirin in pharmacological doses does not interfere with either basal release rates of renin, nor the response to B agonists. It is also suggested that B agonists do not exert their effect by stimulating prostaglandin secretion.