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A role for Ia antigens in thymocyte binding by macrophages

dc.contributor.authorAgrwal, Neeraen_US
dc.contributor.authorThomas, David W.en_US
dc.date.accessioned2006-04-07T18:29:25Z
dc.date.available2006-04-07T18:29:25Z
dc.date.issued1984-04-01en_US
dc.identifier.citationAgrwal, Neera, Thomas, David W. (1984/04/01)."A role for Ia antigens in thymocyte binding by macrophages." Cellular Immunology 84(2): 352-360. <http://hdl.handle.net/2027.42/24858>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WCF-4F800V5-32/2/f1992b7f29c97bd17d00aa162b9b93e1en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/24858
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6608411&dopt=citationen_US
dc.description.abstractTo investigate the membrane structures involved in cellular interactions between thymocytes and macrophages, the relative ability of different murine macrophage populations to spontaneously bind thymocytes was compared. Macrophages derived from the spleen or thymus bound three to four times the number of thymocytes than macrophages from peripheral blood, peritoneum, or bone marrow. This reflects differences both in the number of macrophages binding thymocytes and in the number of thymocytes bound per macrophage. The extent of binding seems to positively correlate with the number of Ia-positive macrophages contained in these populations, as based on previously published values. This was confirmed by showing that elimination of splenic Ia-positive macrophages with anti-Ia and complement treatment dramatically reduced thymocyte binding. In addition, mouse peritoneal washout macrophages incubated for several days with supernatant fluid from concanavalin A-stimulated spleen cells, which induce Ia-antigen expression, exhibited a marked increase in the number of macrophages that bound thymocytes and the number of thymocytes bound per macrophage. To determine if Ia antigens were directly involved in binding, spleen, thymus, or Ia-induced peritoneal macrophages were treated with a monoclonal anti-Ia antibody prior to the addition of thymocytes. Treatment with anti-Ia reduced binding by around 50%, whereas treatment with anti-H-2D antibody had no effect. Monoclonal anti-I-A and anti-I-E antibody treatments of macrophages both inhibited thymocyte binding to similar extents, and treatment of macrophages with both reagents together reduced thymocyte binding by 80%. These results indicate that thymocyte binding is in part dependent on macrophage Ia expression.en_US
dc.format.extent661015 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleA role for Ia antigens in thymocyte binding by macrophagesen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USAen_US
dc.identifier.pmid6608411en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/24858/1/0000285.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0008-8749(84)90107-2en_US
dc.identifier.sourceCellular Immunologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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