The role of a novel VH sequence (V11) in the formation of anti-phosphocholine antibodies
dc.contributor.author | Pierce, Jacqueline W. | en_US |
dc.contributor.author | Hudak, Susan | en_US |
dc.contributor.author | Claflin, J. Latham | en_US |
dc.date.accessioned | 2006-04-07T18:31:51Z | |
dc.date.available | 2006-04-07T18:31:51Z | |
dc.date.issued | 1984-02 | en_US |
dc.identifier.citation | Pierce, Jacqueline W., Hudak, Susan, Claflin, J. Latham (1984/02)."The role of a novel VH sequence (V11) in the formation of anti-phosphocholine antibodies." Molecular Immunology 21(2): 159-166. <http://hdl.handle.net/2027.42/24923> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T9R-476MS6V-NB/2/60b7eaf140b978cec76d5ba984d276f6 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/24923 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6423973&dopt=citation | en_US |
dc.description.abstract | The immune response to phosphocholine (PC) in mice is highly restricted. Most anti-PC antibodies use heavy-chain variable-region (VH) sequences derived from single VH gene segment, V1. In order to investigate whether a highly homologous VH gene segment, V11, could contribute to the formation of PC-binding antibodies, we carried out chain recombination experiments with M47A, a non-PC binding myeloma protein whose H-chain is encoded by the V11 gene segment, and two PC-binding antibodies, HP101.6G6 (HP6G6) and M511. The H-chains from the non-PC-binding myeloma protein, M47A, formed a functional PC-binding site when paired with L-chains from both PC-binding antibodies. These results suggest that a second VH gene segment, V11, could theoretically be used to form PC-binding antibodies. In addition, these results provide direct evidence that a single H-chain can be used in combinatorial association with different L-chains to form antibodies of differing specificities. | en_US |
dc.format.extent | 1198065 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | The role of a novel VH sequence (V11) in the formation of anti-phosphocholine antibodies | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Program in Cellular and Molecular Biology and Department of Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Program in Cellular and Molecular Biology and Department of Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Program in Cellular and Molecular Biology and Department of Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 6423973 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/24923/1/0000350.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0161-5890(84)90131-7 | en_US |
dc.identifier.source | Molecular Immunology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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