Novel shuttle plasmid vehicles for Escherichia-Streptococcus transgeneric cloning

Marcrina, Francis L.; Evans, R. Paul; Tobian, Janet Ash; Hartley, Donna L.; Clewell, Don B.; Jones, Kevin R.

1983-11

Citation: Marcrina, Francis L., Evans, R. Paul, Tobian, Janet Ash, Hartley, Donna L., Clewell, Don B., Jones, Kevin R. (1983/11)."Novel shuttle plasmid vehicles for Escherichia-Streptococcus transgeneric cloning." Gene 25(1, Part 1): 145-150. <http://hdl.handle.net/2027.42/25067>

Abstract: A novel plasmid vector that is able to replicate both in Escherichia coli and in Streptococcus sanguis is described. This 9.2-kb plasmid, designated pVA856, carries Cmr, Tcr and Emr determinants that are expressed in E. coli. Only the Emr determinant is expressed in S. sanguis. Both the Cmr and the Tcr of pVA856 may be insertionally inactivated. This plasmid affords several different cleavage-ligation strategies for cloning in E. coli followed by subsequent introduction of chimeras in to S. sanguis. In addition, we have modified a previously described E. coli-S. sanguis shuttle plasmid [pVA838; Macrina et al., Gene 19 (1982) 345-353], so that it is unable to replicate in S. sanguis. The utility of such a plasmid for cloning and selecting sequences enabling autonomous replication in S. sanguis is demonstrated.

DOIs: http://dx.doi.org/10.1016/0378-1119(83)90176-2

PMID: 6319229

URI: http://www.sciencedirect.com/science/article/B6T39-47T1J7Y-T/2/1acf5179cfeb4563a8bdedfe06f77e25
http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6319229&dopt=citation

Handle: http://hdl.handle.net/2027.42/25067

Show full item record