View Item 

Show simple item record

Novel shuttle plasmid vehicles for Escherichia-Streptococcus transgeneric cloning

dc.contributor.authorMarcrina, Francis L.en_US
dc.contributor.authorEvans, R. Paulen_US
dc.contributor.authorTobian, Janet Ashen_US
dc.contributor.authorHartley, Donna L.en_US
dc.contributor.authorClewell, Don B.en_US
dc.contributor.authorJones, Kevin R.en_US
dc.date.accessioned2006-04-07T18:37:09Z
dc.date.available2006-04-07T18:37:09Z
dc.date.issued1983-11en_US
dc.identifier.citationMarcrina, Francis L., Evans, R. Paul, Tobian, Janet Ash, Hartley, Donna L., Clewell, Don B., Jones, Kevin R. (1983/11)."Novel shuttle plasmid vehicles for Escherichia-Streptococcus transgeneric cloning." Gene 25(1, Part 1): 145-150. <http://hdl.handle.net/2027.42/25067>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T39-47T1J7Y-T/2/1acf5179cfeb4563a8bdedfe06f77e25en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/25067
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6319229&dopt=citationen_US
dc.description.abstractA novel plasmid vector that is able to replicate both in Escherichia coli and in Streptococcus sanguis is described. This 9.2-kb plasmid, designated pVA856, carries Cmr, Tcr and Emr determinants that are expressed in E. coli. Only the Emr determinant is expressed in S. sanguis. Both the Cmr and the Tcr of pVA856 may be insertionally inactivated. This plasmid affords several different cleavage-ligation strategies for cloning in E. coli followed by subsequent introduction of chimeras in to S. sanguis. In addition, we have modified a previously described E. coli-S. sanguis shuttle plasmid [pVA838; Macrina et al., Gene 19 (1982) 345-353], so that it is unable to replicate in S. sanguis. The utility of such a plasmid for cloning and selecting sequences enabling autonomous replication in S. sanguis is demonstrated.en_US
dc.format.extent605623 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleNovel shuttle plasmid vehicles for Escherichia-Streptococcus transgeneric cloningen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelGeneticsen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartments of Oral Biology, and Microbiology and Immunology, Dental Research Institute, University of Michigan, Schools of Medicine and Dentistry, Ann Arbor, MI 48109, U.S.Aen_US
dc.contributor.affiliationotherDepartment of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA23298, U.S.A. Tel. (804) 786-9745en_US
dc.contributor.affiliationotherDepartment of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA23298, U.S.A. Tel. (804) 786-9745en_US
dc.contributor.affiliationotherDepartment of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA23298, U.S.A. Tel. (804) 786-9745en_US
dc.contributor.affiliationotherDepartment of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA23298, U.S.A. Tel. (804) 786-9745en_US
dc.contributor.affiliationotherDepartment of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA23298, U.S.A. Tel. (804) 786-9745en_US
dc.identifier.pmid6319229en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/25067/1/0000498.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0378-1119(83)90176-2en_US
dc.identifier.sourceGeneen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
0000498.pdf
Size:
591.4KB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.