Augmentation of keratinocyte differentiation by the epidermal mitogen, 8-bromo-cAMP
dc.contributor.author | Tong, Philip S. L. | en_US |
dc.contributor.author | Marcelo, Cynthia L. | en_US |
dc.date.accessioned | 2006-04-07T18:37:15Z | |
dc.date.available | 2006-04-07T18:37:15Z | |
dc.date.issued | 1983-11 | en_US |
dc.identifier.citation | Tong, Philip S. L., Marcelo, Cynthia L. (1983/11)."Augmentation of keratinocyte differentiation by the epidermal mitogen, 8-bromo-cAMP." Experimental Cell Research 149(1): 215-226. <http://hdl.handle.net/2027.42/25070> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WFC-4DKKFKK-47/2/0918bfd6f64feb16e22dab2e8d240596 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25070 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6196214&dopt=citation | en_US |
dc.description.abstract | The effect of the epidermal mitogen, 8-bromo-cAMP, on keratinocyte differentiation was studied. A 3 x 10-4 M dose of 8-bromo-cAMP was added to primary neonatal mouse epidermal keratinocyte cultures that slowly proliferate, stratify and differentiate over 2-3 weeks time. [3H]Thymidine autoradiography coupled with an NH4Cl plus reducing agent technic which separates basal and differentiating keratinocytes was used to determine the target cell for the 8-bromo-cAMP mitogenic effect. A histologic stain and a four buffer protein extraction protocol, in conjunction with PAGE and fluorographic technics, were used to assess the differentiation of the cultures. The data indicated that 8-bromo-cAMP primarily stimulated the proliferation of the basal cell monolayer. Simultaneous with the mitogenic effect was an increase in the production of keratohyalin granule, keratin and cell envelope proteins, which are specific markers of epidermal differentiation. The results indicate that keratinocytes stimulated by the epidermal mitogen 8-bromo-cAMP simultaneously express differentiation-related processes. | en_US |
dc.format.extent | 7870947 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Augmentation of keratinocyte differentiation by the epidermal mitogen, 8-bromo-cAMP | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Dermatology, University of Michigan Medical School, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Department of Dermatology, University of Michigan Medical School, Ann Arbor, MI 48109, USA | en_US |
dc.identifier.pmid | 6196214 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25070/1/0000501.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0014-4827(83)90393-2 | en_US |
dc.identifier.source | Experimental Cell Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.