Purification and structural studies of rabbit erythrocyte cytochrome b5
dc.contributor.author | Schafer, Dorothy A. | en_US |
dc.contributor.author | Hultquist, Donald E. | en_US |
dc.date.accessioned | 2006-04-07T18:38:37Z | |
dc.date.available | 2006-04-07T18:38:37Z | |
dc.date.issued | 1983-09-30 | en_US |
dc.identifier.citation | Schafer, Dorothy A., Hultquist, Donald E. (1983/09/30)."Purification and structural studies of rabbit erythrocyte cytochrome b5." Biochemical and Biophysical Research Communications 115(3): 807-813. <http://hdl.handle.net/2027.42/25110> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4G0VTNW-13D/2/f8f8b7c941341b692e44d42e4e057a78 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25110 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6626224&dopt=citation | en_US |
dc.description.abstract | SummaryA single form of cytochrome b5 has been isolated in highly purified form from the cytosolic fraction of rabbit erythrocytes by sequential chromatography on DE-52 cellulose, Sephadex G-75, and DEAE-Sephadex A50. The cytochrome is structurally similar to the N-terminal, heme-binding domain of rabbit liver microsomal cytochrome b5. Like the liver protein, it is blocked at the amino terminus. Its amino acid composition is similar to that of residues 1-97 of the microsomal protein. With one exception, tryptic peptides derived from apo-cytochrome b5 of rabbit erythrocytes co-elute with the tryptic peptides obtained from a soluble hemepeptide fragment of microsomal cytochrome b5. These findings, together with amino acid sequence analysis of the carboxyl terminal tryptic peptides, identify the erythrocyte cytochrome b5 as a 97-residue peptide. | en_US |
dc.format.extent | 444356 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Purification and structural studies of rabbit erythrocyte cytochrome b5 | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry The University of Michigan Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry The University of Michigan Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 6626224 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25110/1/0000542.pdf | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.