Binding of hydrophobic ligands to plant lectins: Titration with arylaminonaphthalenesulfonates
dc.contributor.author | Roberts, David D. | en_US |
dc.contributor.author | Goldstein, Irwin J. | en_US |
dc.date.accessioned | 2006-04-07T18:40:27Z | |
dc.date.available | 2006-04-07T18:40:27Z | |
dc.date.issued | 1983-07-15 | en_US |
dc.identifier.citation | Roberts, David D., Goldstein, Irwin J. (1983/07/15)."Binding of hydrophobic ligands to plant lectins: Titration with arylaminonaphthalenesulfonates." Archives of Biochemistry and Biophysics 224(2): 479-484. <http://hdl.handle.net/2027.42/25163> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WB5-4DW3CSF-13N/2/ea7396229a609e9d955a5b13214e7383 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25163 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6870273&dopt=citation | en_US |
dc.description.abstract | Binding of the Hydrophobic ligands 1,8-anilinonaphthalenesulfonic acid (ANS) and 2,6-toluidinylnaphthalenesulfonic acid (TNS) to a variety of plant lectins was studied by lectin-induced alteration of the fluorescence spectra of the two ligands. With one exception, all legume lectins examined bound ANS, with affinity constants ranging from 103 to 104 M-1. Similar ANS binding was noted for some nonlegume lectins. Titration of the five isolectins from Phaseolus vulgaris with ANS indicated positive cooperative binding of ANS to the two isolectins E4 and E3L1. Titrations with TNS revealed high-affinity sites for this ligand in a number of lectins. Addition of haptenic sugars did not inhibit binding of ANS, suggesting that the hydrophobic binding sites of lectins are independent of the carbohydrate binding sites. | en_US |
dc.format.extent | 513007 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Binding of hydrophobic ligands to plant lectins: Titration with arylaminonaphthalenesulfonates | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 6870273 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25163/1/0000599.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-9861(83)90235-7 | en_US |
dc.identifier.source | Archives of Biochemistry and Biophysics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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