Effect of immunosuppressive agents on human T and B lymphoblasts
dc.contributor.author | Kazmers, Irene S. | en_US |
dc.contributor.author | Daddona, Peter E. | en_US |
dc.contributor.author | Dalke, A. Paulette | en_US |
dc.contributor.author | Kelley, William N. | en_US |
dc.date.accessioned | 2006-04-07T18:45:22Z | |
dc.date.available | 2006-04-07T18:45:22Z | |
dc.date.issued | 1983-03-01 | en_US |
dc.identifier.citation | Kazmers, Irene S., Daddona, Peter E., Dalke, A. Paulette, Kelley, William N. (1983/03/01)."Effect of immunosuppressive agents on human T and B lymphoblasts." Biochemical Pharmacology 32(5): 805-810. <http://hdl.handle.net/2027.42/25295> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T4P-478BHVX-2B/2/02adba8e98ff6a93654e3d2c1747757a | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25295 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6404281&dopt=citation | en_US |
dc.description.abstract | We have studied the effects of various immunosuppressive drugs on the growth of human-derived T (MOLT-4) and B (MGL-8) lymphoblasts. In addition, we have examined whether the lymphotoxic effect of any of these drugs could be attributed to inhibition of either adenosine deaminase (DDA) or purine nucleoside phosphorylase (PNP). Results indicated that 1-[beta]--arabinofuranosylcytosine (Ara-C), methotrexate and chlorambucil were four to seven times more toxic for T than for B cells, while azathioprine, 6-thioguanine, 6-mercaptopurine, and 5-fluorouracil were highly toxic for both T and B cells. Cyclophosphamide and oxisuran were lymphotoxic only at concentrations exceeding 300 [mu]M. Deoxyadenosine (50 [mu]M), deoxyguanosine (10 [mu]M) and deoxycoformycin (10 [mu]M) failed to enhance T cell toxicity when individually combined with each drug. None of the drugs tested inhibited T or B lymphoblast ADA or PNP activity. With the exception of Ara-C, neither dATP nor dGTP accumulated in T lymphoblasts incubated in the presence of any of the drugs. We conclude that the cell culture system used in this investigation is useful for identifying lymphotoxic and T cell-specific immunosuppressive agents. However, none of the drugs studied appeared to function as an inhibitor of, or a competitive substrate for, either ADA or PNP. | en_US |
dc.format.extent | 560343 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Effect of immunosuppressive agents on human T and B lymphoblasts | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, MI 48109, U.S.A.; Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, MI 48109, U.S.A.; Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, MI 48109, U.S.A.; Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, MI 48109, U.S.A.; Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 6404281 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25295/1/0000738.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-2952(83)90580-4 | en_US |
dc.identifier.source | Biochemical Pharmacology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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