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Modulation of Ia and photoreactive antigen on antigen-presenting cells: Fun with a photoprobe

dc.contributor.authorThomas, David W.en_US
dc.contributor.authorEades, Linda J.en_US
dc.contributor.authorWilson, Cherylen_US
dc.contributor.authorSolvay, Maxine J.en_US
dc.date.accessioned2006-04-07T18:53:31Z
dc.date.available2006-04-07T18:53:31Z
dc.date.issued1985-12en_US
dc.identifier.citationThomas, David W., Eades, Linda, Wilson, Cheryl, Solvay, Maxine J. (1985/12)."Modulation of Ia and photoreactive antigen on antigen-presenting cells: Fun with a photoprobe." Cellular Immunology 96(2): 351-362. <http://hdl.handle.net/2027.42/25478>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WCF-4F686TR-64/2/8927bcb1a19688b89ddf80c6449286e2en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/25478
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2427213&dopt=citationen_US
dc.description.abstractTo identify the antigen-specific recognition complex containing elements from T cells and antigen-presenting cells (APC), a photoactivatable antigen system was developed which could potentially crosslink the complex during the specific cellular responses. In this paper we describe the development of this system using murine T-cell hybridomas responding to stimulator cells chemically conjugated with N-hydroxysuccinimidyl 4-azidobenzoate (HSAB) and genetically restricted by I-Ad. In initial experiments it was found that several I-Ad-positive B-cell lines were nonstimulatory when coupled with HSAB, but that I-Ad-positive P388D1 macrophage-like cells were efficient stimulators of HSAB-specific T-cell responses. These results suggested that the relevant HSAB coupled surface structure was not likely I-Ad. To substantiate this point, Ia-positive or Ia-negative P388D1 cells were initially coupled with HSAB and the expression of Ia was modulated by the addition and withdrawal of Con A-stimulated spleen cell supernatant fluid through several days of culture. Under these conditions, efficient stimulation was only observed when Ia was expressed, although the HSAB antigen was continuously present. In other experiments it was found that exposure of HSAB-coupled APC to light selectively eliminated their stimulatory capacity for HSAB-specific T hybridomas, suggesting that the light-induced crosslinking by HSAB directly eliminates the antigenic determinant. This antigen system allows a unique opportunity to manipulate the antigen during specific cellular interactions, and to introduce covalent crosslinking of the specific antigen recognition complex that may allow its isolation and characterization.en_US
dc.format.extent950295 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleModulation of Ia and photoreactive antigen on antigen-presenting cells: Fun with a photoprobeen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor, Michigan 48109-0010, USAen_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor, Michigan 48109-0010, USAen_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor, Michigan 48109-0010, USAen_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor, Michigan 48109-0010, USAen_US
dc.identifier.pmid2427213en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/25478/1/0000018.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0008-8749(85)90366-1en_US
dc.identifier.sourceCellular Immunologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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