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Analysis of plasma cyanate as 2-nitro-5-thiocarbamylbenzoic acid by high-performance liquid chromatography

dc.contributor.authorEiger, Steven M.en_US
dc.contributor.authorBlack, Shaun D.en_US
dc.date.accessioned2006-04-07T19:05:26Z
dc.date.available2006-04-07T19:05:26Z
dc.date.issued1985-05-01en_US
dc.identifier.citationEiger, Steven, Black, Shaun D. (1985/05/01)."Analysis of plasma cyanate as 2-nitro-5-thiocarbamylbenzoic acid by high-performance liquid chromatography." Analytical Biochemistry 146(2): 321-326. <http://hdl.handle.net/2027.42/25681>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6W9V-4DX48JX-JB/2/2a64311f6736c13c8fc0adb792ec2b61en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/25681
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4025800&dopt=citationen_US
dc.description.abstractA method for the determination of cyanate concentration in blood plasma over the range 1 to 1000 [mu] is presented. Cyanate present in the dried residue of acetone-deproteinized plasma is converted to a chromophoric thiocarbamyl derivative by addition of pH 3.0-buffered thionitrobenzoic acid. The derivative is the analyzed by reversed-phase high-performance liquid chromatography with detection at 313 nm, near the absorption maximum. Carbamyl thionitrobenzoic acid peak height is quantified by comparison to a standard curve made by analysis of plasma samples to which known quantities of cyanate have been added. This technique is sensitive and linear with respect to cyanate concentration, and is faster than other reported methods; sample analysis and column regereration are accomplished within 20 min.en_US
dc.format.extent489063 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleAnalysis of plasma cyanate as 2-nitro-5-thiocarbamylbenzoic acid by high-performance liquid chromatographyen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109, USAen_US
dc.identifier.pmid4025800en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/25681/1/0000235.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0003-2697(85)90546-9en_US
dc.identifier.sourceAnalytical Biochemistryen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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