A deterministic approach for the estimation of mutation rates in cultured mammalian cells
dc.contributor.author | Li, I-Chian | en_US |
dc.contributor.author | Ho Wu, Shu-Chen | en_US |
dc.contributor.author | Fu, Jiliang | en_US |
dc.contributor.author | Chu, Ernest H. Y. | en_US |
dc.date.accessioned | 2006-04-07T19:08:17Z | |
dc.date.available | 2006-04-07T19:08:17Z | |
dc.date.issued | 1985-03 | en_US |
dc.identifier.citation | Li, I-Chian, Ho Wu, Shu-Chen, Fu, Jiliang, Chu, E. H. Y. (1985/03)."A deterministic approach for the estimation of mutation rates in cultured mammalian cells." Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 149(1): 127-132. <http://hdl.handle.net/2027.42/25744> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T2C-47MKK96-K6/2/69a8399b7486554a339315673b7f98b2 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25744 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3974619&dopt=citation | en_US |
dc.description.abstract | Unequal growth rates between mutant and wild-type cells in a large population constitute a problem for the estimation of mutation rate. Over a period of cell growth, a selective advantage of one cell type over the other might lead to considerable error in the estimation of mutation rate if equal growth rates are assumed. In this study, we propose a formula and apply it to the estimation of spontaneous mutation rate in a growing population of Chinese hamster V79 cells in which ouabain-resistant mutant cells exhibit a slower growth rate than the wild-type cells. The formula is a generalization of that previously presented by Armitage (1953), and this is the first attempt to apply the deterministic approach for mutation rate estimation to cultured mammalian cells. The value of the estimated rate is compred with that derived from a parallel experiment using the fluctuation test of Luria and Delbruck (1943). The limitations and advantages of taking the deterministic approach to mutation rate estimation in mammalian cell systems are discussed. | en_US |
dc.format.extent | 455655 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | A deterministic approach for the estimation of mutation rates in cultured mammalian cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, Medical School, School of Public Health, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Biostatistics, School of Public Health, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Human Genetics, Medical School, School of Public Health, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Human Genetics, Medical School, School of Public Health, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 3974619 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25744/1/0000304.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0027-5107(85)90017-X | en_US |
dc.identifier.source | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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