Progastrin-like immunoreactivity in porcine antrum: Identification and characterization with region-specific antisera
dc.contributor.author | Sugano, Kentaro | en_US |
dc.contributor.author | Yamada, Tadataka | en_US |
dc.date.accessioned | 2006-04-07T19:10:28Z | |
dc.date.available | 2006-04-07T19:10:28Z | |
dc.date.issued | 1985-01-16 | en_US |
dc.identifier.citation | Sugano, Kentaro, Yamada, Tadataka (1985/01/16)."Progastrin-like immunoreactivity in porcine antrum: Identification and characterization with region-specific antisera." Biochemical and Biophysical Research Communications 126(1): 72-77. <http://hdl.handle.net/2027.42/25781> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4DYM9YK-WF/2/aa19b7bee48766b8abf6cce567d340d6 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/25781 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3838247&dopt=citation | en_US |
dc.description.abstract | In an effort to identify and characterize precursors of gastrin in tissues, we generated region-specific antisera against a synthetic progastrin peptide, Tyr-Gly-Trp-Met-Asp-Phe-Gly-Arg-Arg (GL9), as deduced from the nucleotide sequence of gastrin mRNA. This antisera did not cross-react with gastrin or progastrin peptides with shorter carboxyl-terminal extensions. Progastrin-like immunoreactivity (PGLI) was measured in porcine antrum at a concentration of 6.8 +/- 1.2 pmol/g wet weight (mean +/- SE, N = 5), or roughly 0.2% of that of gastrin. On Sephadex G50 chromatography, a major peak of PGLI was eluted as a slightly larger molecule than gastrin heptadecapeptide (G17) but possessed the same N-terminal immunoreactivity. These findings suggest that G17 may be formed by processing of a carboxyl-terminally extended precursor as an alternative to cleavage of big gastrin (G34). | en_US |
dc.format.extent | 340702 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Progastrin-like immunoreactivity in porcine antrum: Identification and characterization with region-specific antisera | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, Michigan, USA | en_US |
dc.identifier.pmid | 3838247 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/25781/1/0000342.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-291X(85)90572-8 | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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