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Measurement of immunoglobulin concentration in cell culture supernates by computer-assisted ELISA

dc.contributor.authorSlade, Herbert B.en_US
dc.contributor.authorLopatin, Dennis E.en_US
dc.contributor.authorSchwartz, Stanley A.en_US
dc.date.accessioned2006-04-07T19:24:00Z
dc.date.available2006-04-07T19:24:00Z
dc.date.issued1986-11-20en_US
dc.identifier.citationSlade, Herbert B., Lopatin, Dennis E., Schwartz, Stanley A. (1986/11/20)."Measurement of immunoglobulin concentration in cell culture supernates by computer-assisted ELISA." Journal of Immunological Methods 94(1-2): 169-179. <http://hdl.handle.net/2027.42/25976>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T2Y-476KRNP-CC/2/3672ae06da9cde552c689ee16f09e538en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/25976
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3782809&dopt=citationen_US
dc.description.abstractThe enzyme-linked immunosorbent assay (ELISA) is used extensively in immunologic research for obtaining quantitative estimates of immunoglobulin concentration in cell culture supernates. Through incorporation of a microcomputer for data acquisition, storage and rapid calculation of results, a substantial reduction in total assay time may be realized. Described here are a set of menu-driven programs written in Basic for the IBM-PC which provide advantages over existing software in simplicity, versatility and accuracy. Hardware requirements are minimal. These programs should encourage greater flexibility in terms of the size and complexity of experimental designs.en_US
dc.format.extent652989 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleMeasurement of immunoglobulin concentration in cell culture supernates by computer-assisted ELISAen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumImmunology Section, Dept. of Pediatrics, 3081 SPH-I, University of Michigan, Dental Research Institute, 1192 SE, 300 N. Ingalls St., University of Michigan, Ann Arbor, MI 48109, U.S.A.: Depts. of Pediatrics, Epidemiology and Microbiology/Immunology, 3073 SPH-I, University of Michigan, Ann Arbor, MI 48109, U.S.A.en_US
dc.contributor.affiliationumImmunology Section, Dept. of Pediatrics, 3081 SPH-I, University of Michigan, Dental Research Institute, 1192 SE, 300 N. Ingalls St., University of Michigan, Ann Arbor, MI 48109, U.S.A.: Depts. of Pediatrics, Epidemiology and Microbiology/Immunology, 3073 SPH-I, University of Michigan, Ann Arbor, MI 48109, U.S.A.en_US
dc.contributor.affiliationumImmunology Section, Dept. of Pediatrics, 3081 SPH-I, University of Michigan, Dental Research Institute, 1192 SE, 300 N. Ingalls St., University of Michigan, Ann Arbor, MI 48109, U.S.A.: Depts. of Pediatrics, Epidemiology and Microbiology/Immunology, 3073 SPH-I, University of Michigan, Ann Arbor, MI 48109, U.S.A.en_US
dc.identifier.pmid3782809en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/25976/1/0000042.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0022-1759(86)90230-9en_US
dc.identifier.sourceJournal of Immunological Methodsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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