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A rapid method for the isolation of peroxisomes from rat liver

dc.contributor.authorGhosh, Mridul K.en_US
dc.contributor.authorHajra, Amiya K.en_US
dc.date.accessioned2006-04-07T19:24:05Z
dc.date.available2006-04-07T19:24:05Z
dc.date.issued1986-11-15en_US
dc.identifier.citationGhosh, Mridul K., Hajra, Amiya K. (1986/11/15)."A rapid method for the isolation of peroxisomes from rat liver." Analytical Biochemistry 159(1): 169-174. <http://hdl.handle.net/2027.42/25978>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6W9V-4DYM9HN-14X/2/e99b23767467ce3f5284113714acd525en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/25978
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3812996&dopt=citationen_US
dc.description.abstractA preparative method for the isolation of peroxisomes from the liver of normal, untreated rats is described. The peroxisome-enriched "light mitochondrial" fraction is layered on a 30% Nycodenz (5-[N-2,3-dihydroxypropylacetamido]-2,4,6-triiodo-N,N'-bis[2,3-dihydroxypropyl]isophthalamide) solution containing 1 m tetrasodium EDTA and then centrifuged in an angular rotor for 1 h at 130,000gavg. Peroxisomes are sedimented to the bottom leaving other organelles at the top of the tube. On the basis of morphological and biochemical studies, it is found that the peroxisomes (marker-enzymes catalase and urate oxidase) obtained in this method are not contaminated with lysosomes (marker-enzyme acid phosphatase) and contained very few mitochondria (marker-enzyme succinate-cytochrome c reductase) and microsomal vesicles (marker-enzyme glucose-6-phosphatase).en_US
dc.format.extent2378503 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleA rapid method for the isolation of peroxisomes from rat liveren_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry, University of Michigan, 1103 East Huron, Ann Arbor, Michigan 48109, USA: Neuroscience Laboratory, Mental Health Research Institute, University of Michigan, 1103 East Huron, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumNeuroscience Laboratory, Mental Health Research Institute, University of Michigan, 1103 East Huron, Ann Arbor, Michigan 48109, USA: Department of Biological Chemistry, University of Michigan, 1103 East Huron, Ann Arbor, Michigan 48109, USAen_US
dc.identifier.pmid3812996en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/25978/1/0000044.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0003-2697(86)90323-4en_US
dc.identifier.sourceAnalytical Biochemistryen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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