Isolation, stages of differentiation, and long term maintenance of adult rat myocytes
dc.contributor.author | Weishaar, Ronald E. | en_US |
dc.contributor.author | Simpson, Robert U. | en_US |
dc.date.accessioned | 2006-04-07T19:27:13Z | |
dc.date.available | 2006-04-07T19:27:13Z | |
dc.date.issued | 1986-09 | en_US |
dc.identifier.citation | Weishaar, Ronald E., Simpson, Robert U. (1986/09)."Isolation, stages of differentiation, and long term maintenance of adult rat myocytes." Cell Biology International Reports 10(9): 745-753. <http://hdl.handle.net/2027.42/26066> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B7MF6-4DYN475-CP/2/8377e53fac724b16ad20af66af5a2d7d | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26066 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3757069&dopt=citation | en_US |
dc.description.abstract | Although a variety of techniques have been developed to isolate myocytes from adult hearts, the long term viability of such cells has only recently been investigated. In addition, relatively little is known about the stages of differentiation such cells proceed through following isolation. In the present study myocytes were isolated using two techniques, one involving retrograde perfusion via the aorta, and the other involving mechanical "shearing." In addition, several modifications were made to minimize the trauma normal associated with isolating myocytes from adult hearts. Both techniques yielded a high percentage of rod-shaped, quiescent myocytes, although myocytes isolated using the "shearing" method were less likely to remain viable for more than 24 hours. With both techniques those cells which remained viable for more than 24 hours proceeded through an identical pattern of differentiation leading to stable, attached cells which remained viable for up to four weeks. These results demonstrate that with the appropriate isolation techniques it is possible to maintain adult myocardial cells in culture for lengthy periods of time. | en_US |
dc.format.extent | 4754818 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Isolation, stages of differentiation, and long term maintenance of adult rat myocytes | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan, Medical Science I, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan, Medical Science I, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.identifier.pmid | 3757069 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26066/1/0000140.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0309-1651(86)90132-3 | en_US |
dc.identifier.source | Cell Biology International Reports | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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