Immunochemical studies on the combining site of the blood group A-special lima bean lectin

Abstract

The combining site of the lima bean (Phaseolus lunatus) lectin (LBL) was studied by quantitative precipitin and precipitin-inhibition assays. The lectin precipitated best with hog gastric mucosa and human ovarian cyst blood group A1 substances and moderately with A2 substances. B substances precipitated very poorly and H, Lea, Leb, and precursor I substances did not react. Blood group A1 and A2 substances reacted to varying extents and these differences are attributable to heterogeneity resulting from incomplete biosynthesis of carbohydrate chains. By inhibition of precipitation of LBL with A1 blood group substance, the lectin was found to be most specific for fucose-containing oligosaccharides having the A trisaccharide, GalNAc[alpha]1-->3[-Fuc[alpha]1-->2]Gal determinant. The best inhibitor, an A-specific hexasaccharide, GalNAc[alpha]1-->3[Fuc[alpha]1-->2]Gal[beta]1-->3GlcNAc[beta]1-->3-Gal[beta]1-->4Glc, was 11 times more active than the A trisaccharide. A difucosyl oligosaccharide with a second fucose linked [alpha]1-->3 to the GlcNAc is less active; fucose linked [alpha]1-->4 to GlcNAc was completely inactive. These results suggest that specific interactions with the subterminal sugars may be important in the binding, and that the specificity of the lectin combining site involves at least the nonreducing terminal four and probably five sugars of the hexasaccharide. Thus LBL has a more-extended binding site than was inferred previously and is in the upper range of antibody combining-site sizes.