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Isolation of galactosyltransferase from human milk and the determination of its N-terminal amino acid sequence

dc.contributor.authorAppert, Hubert E.en_US
dc.contributor.authorRutherford, Thomas J.en_US
dc.contributor.authorTarr, George E.en_US
dc.contributor.authorThomford, Neil R.en_US
dc.contributor.authorMcCorquodale, D. Jamesen_US
dc.date.accessioned2006-04-07T19:28:29Z
dc.date.available2006-04-07T19:28:29Z
dc.date.issued1986-07-16en_US
dc.identifier.citationAppert, Hubert E., Rutherford, Thomas J., Tarr, George E., Thomford, Neil R., McCorquodale, D. James (1986/07/16)."Isolation of galactosyltransferase from human milk and the determination of its N-terminal amino acid sequence." Biochemical and Biophysical Research Communications 138(1): 224-229. <http://hdl.handle.net/2027.42/26099>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WBK-4F031HH-MF/2/0425ebd71e3f1c71c5d1427bf9b77539en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/26099
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3091013&dopt=citationen_US
dc.description.abstractGalactosyltransferase (EC 2.4.1.22), purified to homogeneity from human milk by affinity chromatography, had an apparent molecular weight of 53,000 as determined by denaturing polyacrylamide gel electrophoresis. Subtration of the estimated contribution of the oligosaccharide portion of the molecule leaves a Mr of 47,000. An N-terminal amino acid sequence analysis of the isolated protein revealed a sequence similar to that found near the 5' end of a cDNA clone isolated by Shaper et al (11), which encodes a 35,500 molecular weight protein. Either the molecular weight of galactosyltransferase, has been overestimated, or a discrepancy exists between the actual molecular weight of galactosyltransferase and that predicted by the bovine cDNA clone isolated by Shaper et al (11).en_US
dc.format.extent1175715 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleIsolation of galactosyltransferase from human milk and the determination of its N-terminal amino acid sequenceen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumUniversity of Michigan, Department of Biochemistry, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationotherMedical College of Ohio at Toledo, Department of Surgery C.S. 10008, Toledo, Ohio 43699, USAen_US
dc.contributor.affiliationotherMedical College of Ohio at Toledo, Department of Biochemistry C.S. 10008, Toledo, Ohio 43699, USAen_US
dc.contributor.affiliationotherMedical College of Ohio at Toledo, Department of Surgery C.S. 10008, Toledo, Ohio 43699, USAen_US
dc.contributor.affiliationotherMedical College of Ohio at Toledo, Department of Biochemistry C.S. 10008, Toledo, Ohio 43699, USAen_US
dc.identifier.pmid3091013en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/26099/1/0000175.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0006-291X(86)90269-Xen_US
dc.identifier.sourceBiochemical and Biophysical Research Communicationsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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