Purification and properties of an oxygen-evolving reaction center complex from photosystem II membranes : A simple procedure utilizing a non-ionic detergent and elevated ionic strength
dc.contributor.author | Ghanotakis, Demetrios F. | en_US |
dc.contributor.author | Yocum, Charles F. | en_US |
dc.date.accessioned | 2006-04-07T19:33:27Z | |
dc.date.available | 2006-04-07T19:33:27Z | |
dc.date.issued | 1986-03-03 | en_US |
dc.identifier.citation | Ghanotakis, Demetrios F., Yocum, Charles F. (1986/03/03)."Purification and properties of an oxygen-evolving reaction center complex from photosystem II membranes : A simple procedure utilizing a non-ionic detergent and elevated ionic strength." FEBS Letters 197(1-2): 244-248. <http://hdl.handle.net/2027.42/26236> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T36-449T4DS-GK/2/5845234f55f4001af803dd56d110d9db | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26236 | |
dc.description.abstract | A method is reported for the isolation of a highly resolved oxygen-evolving photosystem II reaction center preparation. This preparation can be separated from the more complex photosystem II membranes isolated by the procedure of Berthold et al. [(1981) FEBS Lett. 134, 231-234] by use of octylglucopyranoside at elevated ionic strengths; the oxygen-evolving material can be collected by centrifugation at relatively low g values (40000 x g) in yields estimated to be more than 80%. This new preparation lacks the 17 and 23 kDa extrinsic polypeptides; addition of calcium and chloride produces activities approaching 1000 [mu]mol O2/h per mg chlorophyll. Although activity is maximal in the presence of 2,5-dichloro-[beta]-benzoquinone, the response of activity to ferricyanide and 3-(3,4-dichlorophenyl)-1,1-dimethylurea indicates that the reducing side of photosystem II has been modified in this new oxygen-evolving reaction center preparation. | en_US |
dc.format.extent | 466662 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Purification and properties of an oxygen-evolving reaction center complex from photosystem II membranes : A simple procedure utilizing a non-ionic detergent and elevated ionic strength | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Biological Sciences and Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109-1048, USA | en_US |
dc.contributor.affiliationum | Division of Biological Sciences and Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109-1048, USA | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26236/1/0000316.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0014-5793(86)80335-0 | en_US |
dc.identifier.source | FEBS Letters | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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