Ultrastructural morphology and cytochemistry of iron-deficient polymorphonuclear leukocytes
dc.contributor.author | Parmley, R. T. | en_US |
dc.contributor.author | Barton, J. C. | en_US |
dc.contributor.author | Fittschen, C. | en_US |
dc.contributor.author | Boxer, Laurence A. | en_US |
dc.date.accessioned | 2006-04-07T19:34:34Z | |
dc.date.available | 2006-04-07T19:34:34Z | |
dc.date.issued | 1986-02 | en_US |
dc.identifier.citation | Parmley, R. T., Barton, J. C., Fittschen, C., Boxer, L. A. (1986/02)."Ultrastructural morphology and cytochemistry of iron-deficient polymorphonuclear leukocytes." Experimental and Molecular Pathology 44(1): 57-66. <http://hdl.handle.net/2027.42/26267> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WFB-4C4NY1P-8S/2/36e2b4167069b12fef036ea8f16bca0e | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26267 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3948978&dopt=citation | en_US |
dc.description.abstract | Previous studies have documented decreased activities of certain enzymes and altered function in polymorphonuclear leukocytes (PMN) during iron deficiency. The present study was undertaken to determine if the enzymatic abnormalities could be correlated with morphologic or quantitative change in PMN granules. Ultrastructural examination of primary and secondary granules and assessment of the secondary granule components alkaline phosphatase and vicinal glycol-containing glycoconjugates was performed in rabbit bone marrow, peripheral blood, and peritoneal heterophils. In addition, biochemical quantifications of the secondary granule component alkaline phosphatase and the primary granule marker [beta]-glucuronidase were performed. The results confirmed that a marked, significant decrease in alkaline phosphatase occurs in iron-deficient animals; however, no biochemical decrease in [beta]-glucuronidase activity was observed. Ultrastructurally, PMN secondary granules of iron-deficient rabbits tended to be more numerous than in controls when examined with morphometric and glycoconjugate staining methods, but lacked staining in alkaline phosphatase preparations. These results demonstrate that iron-deficient rabbits produce normal to increased quantities of primary and secondary granules, despite a uniform deficiency of alkaline phosphatase, a secondary granule marker. | en_US |
dc.format.extent | 1148798 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Ultrastructural morphology and cytochemistry of iron-deficient polymorphonuclear leukocytes | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Pathology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pediatrics, University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationother | Department of Pediatrics and Pathology, The University of Texas Health Science Center at San Antonio, USA | en_US |
dc.contributor.affiliationother | Department of Medicine, Veterans Administration Hospital, Birmingham, Alabama, USA | en_US |
dc.contributor.affiliationother | Comparative Medicine, University of Alabama at Birmingham, Veterans Administration Hospital, Birmingham, Alabama, USA | en_US |
dc.identifier.pmid | 3948978 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26267/1/0000352.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0014-4800(86)90033-X | en_US |
dc.identifier.source | Experimental and Molecular Pathology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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