(Na+ + K+)-ATPase in C6 glioma and rat cerebrum
dc.contributor.author | Sheedlo, Harold J. | en_US |
dc.contributor.author | Starosta-Rubinstein, Simon | en_US |
dc.contributor.author | Desmond, Timothy J. | en_US |
dc.contributor.author | Siegel, George J. | en_US |
dc.date.accessioned | 2006-04-07T19:52:40Z | |
dc.date.available | 2006-04-07T19:52:40Z | |
dc.date.issued | 1987-06 | en_US |
dc.identifier.citation | Sheedlo, Harold J., Starosta-Rubinstein, Simon, Desmond, Timothy J., Siegel, George J. (1987/06)."(Na+ + K+)-ATPase in C6 glioma and rat cerebrum." Journal of Neuroimmunology 15(2): 173-184. <http://hdl.handle.net/2027.42/26689> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T03-476VRMH-6H/2/85be6b85525306d0eb45ed4d3af367f8 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26689 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3034973&dopt=citation | en_US |
dc.description.abstract | The content and distribution of the membranee-bound enzyme (Na+ + K+)-ATPase in a rat cerebral C6 glioma was determined by immunocytochemistry, immunoblots and enzyme assay. In the C6 glioma cell culture (Na+ + K+)-ATPase activity was about 20% of (Mg2+ + Na+ + K+)-ATPase activity. However, (Mg2+ + Na+ + K+)-ATPase activity in the cerebral C6 gliomas was very close to Mg2+ baseline and not significantly increased by Na+ and K+. As shown by immunobloting, (Na+ + K+)-ATPase catalytic subunit was detected in excised samples of control cerebrum and as a trace in the intracerebral portions of C6 glioma but not at all in the extracranial portions of C6 glioma or in C6 glioma cell culture. (Na+ + K+)-ATPase was not detected immunocytochemically in paraffin sections of the extracranial or intracerebral portions of rat cerebral C6 glioma. The absence of staining for (Na+ + K+)-ATPase clearly demarcated projections of glioma within normal brain. These results suggest that C6 glioma has little if any expression of (Na+ + K+)-ATPase in vitro or in vivo. The small amount of enzyme epitope in the intracerebral portions represents contamination by normal cerebrum in the extracts. | en_US |
dc.format.extent | 820901 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | (Na+ + K+)-ATPase in C6 glioma and rat cerebrum | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Neurology, Neuroscience Laboratory Building, University of Michigan, Ann Arbor, MI 48104, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Neurology, Neuroscience Laboratory Building, University of Michigan, Ann Arbor, MI 48104, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Neurology, Neuroscience Laboratory Building, University of Michigan, Ann Arbor, MI 48104, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Neurology, Neuroscience Laboratory Building, University of Michigan, Ann Arbor, MI 48104, U.S.A. | en_US |
dc.identifier.pmid | 3034973 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26689/1/0000236.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0165-5728(87)90091-9 | en_US |
dc.identifier.source | Journal of Neuroimmunology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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