Protein microinjection by protease permeabilization of fibroblasts
dc.contributor.author | Lemons, Rosemary M. | en_US |
dc.contributor.author | Forster, Susan | en_US |
dc.contributor.author | Thoene, Jess G. | en_US |
dc.date.accessioned | 2006-04-07T20:16:39Z | |
dc.date.available | 2006-04-07T20:16:39Z | |
dc.date.issued | 1988-07 | en_US |
dc.identifier.citation | Lemons, Rosemary, Forster, Susan, Thoene, Jess (1988/07)."Protein microinjection by protease permeabilization of fibroblasts." Analytical Biochemistry 172(1): 219-227. <http://hdl.handle.net/2027.42/27241> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6W9V-4DW2KKR-K6/2/007e9e67df66434ead8f9b3f793cc86f | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/27241 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2847580&dopt=citation | en_US |
dc.description.abstract | Exposure of cultured diploid fibroblasts to protease solutions induces a hyperpermeable state which permits entry of exogenous macromolecules directly into the cytosol. We have exploited this finding to devise a microinjection method whose chief advantages are simplicity and good retention of cell viability. Proteins successfully injected by this technique range from insulin to thyroglobulin. The amounts injected range from 4 x 105 to 5 x 106 molecules/cell. | en_US |
dc.format.extent | 2779862 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Protein microinjection by protease permeabilization of fibroblasts | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, School of Medicine, Ann Arbor, Michigan 48109, USA; Department of Pediatrics, University of Michigan, School of Medicine, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, School of Medicine, Ann Arbor, Michigan 48109, USA; Department of Pediatrics, University of Michigan, School of Medicine, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Pediatrics, University of Michigan, School of Medicine, Ann Arbor, Michigan 48109, USA; Department of Biological Chemistry, University of Michigan, School of Medicine, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 2847580 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/27241/1/0000248.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-2697(88)90435-6 | en_US |
dc.identifier.source | Analytical Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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