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A rapid and sensitive screening method for the detection of anti-2-acetylaminofluorene immunoglobulins

dc.contributor.authorSilbart, Lawrence K.en_US
dc.contributor.authorNordblom, Gerald D.en_US
dc.contributor.authorKeren, David F.en_US
dc.contributor.authorWise, Jr. , Dean S.en_US
dc.contributor.authorLincoln, Pamela M.en_US
dc.contributor.authorTownsend, Leroy B.en_US
dc.date.accessioned2006-04-07T20:20:16Z
dc.date.available2006-04-07T20:20:16Z
dc.date.issued1988-04-22en_US
dc.identifier.citationSilbart, Lawrence K., Nordblom, Gerald, Keren, David F., Wise, Jr., Dean S., Lincoln, Pamela M., Townsend, Leroy B. (1988/04/22)."A rapid and sensitive screening method for the detection of anti-2-acetylaminofluorene immunoglobulins." Journal of Immunological Methods 109(1): 103-112. <http://hdl.handle.net/2027.42/27330>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T2Y-476ND00-11N/2/5b7a18a93e21180d4f4ce4dd2033c7a4en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/27330
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3356906&dopt=citationen_US
dc.description.abstractA method is described in which anti-2-acetylaminofluorene immunoglobulins may be detected using a simple and sensitive screening procedure. The method is based on immunoglobulin binding of an 125I derivatized 2-aminofluorene radiotracer. Tracer binding is not isotype specific, and thus the method is useful for the detection of either IgG or IgA. Competitive binding experiments with the radiotracer were used to determine the specificity of immunoglobulin response by measurement of cross-reactivity with related ligands. This method allows quantitation of the immune response to the carcinogen in serum and other biological fluids (i.e., intestinal secretions).en_US
dc.format.extent759396 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleA rapid and sensitive screening method for the detection of anti-2-acetylaminofluorene immunoglobulinsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pathology, Biochemistry Section, The University of Michigan, Ann Arbor, MI 48109, U.S.A.en_US
dc.contributor.affiliationumDepartment of Pathology, Biochemistry Section, The University of Michigan, Ann Arbor, MI 48109, U.S.A.en_US
dc.contributor.affiliationumDepartment of Medicinal Chemistry, The University of Michigan, Ann Arbor, MI 48109, U.S.A.en_US
dc.contributor.affiliationumDepartment of Pathology, Biochemistry Section, The University of Michigan, Ann Arbor, MI 48109, U.S.A.en_US
dc.contributor.affiliationumDepartment of Medicinal Chemistry, The University of Michigan, Ann Arbor, MI 48109, U.S.A.en_US
dc.contributor.affiliationotherWarner Lambert Corporation, 2800 Plymouth Road, Ann Arbor, MI 48105, U.S.A.en_US
dc.identifier.pmid3356906en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/27330/1/0000354.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0022-1759(88)90447-4en_US
dc.identifier.sourceJournal of Immunological Methodsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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