Responses of lamb nucleus of the solitary tract neurons to chemical stimulation of the epiglottis

Abstract

Previous research has shown that applications of chemical stimuli to the epiglottis produced distinct patterns of activity in the lamb superior laryngeal nerve. To determine the response characteristics of second-order neurons, we recorded from single cells in the lamb nucleus of the solitary tract (NST) while stimulating the epiglottis with 0.5 M KCl, NH4Cl, NaCl, LiCl, distilled water, 0.005 M citric acid and 0.01 N HCl. Most neurons responded to more than one of the chemical solutions. The order of effective stimuli was KCl = NH4Cl > distilled water > HCl > citric acid > NaCl > LiCl. An analysis of the variation in response frequency over time found that different chemical stimuli produced significantly different response patterns in NST neurons. A comparison of the mean neural response profiles of NST neurons and superior laryngeal nerve fibers for each of the stimuli found that only the response profiles elicited by NH4Cl were significantly different. In addition to their responses to chemical solutions, almost one-third of the NST neurons responded to the rinse following application of at least some of the stimuli and 80% of the neurons were excited by mechanical stimulation of the epiglottis with a soft brush. Also, a small number of neurons exhibited a rhythmic response coordinated with respiration. The majority of recording sites were located in areas of the NST linked to swallowing and respiration suggesting that the response patterns of NST neurons elicited by chemical stimulation of receptors on the epiglottis may play a role in upper airway reflexes.