Bacteremia-induced suppression of alveolar surfactant production,
dc.contributor.author | Oldham, Keith T. | en_US |
dc.contributor.author | Guice, Karen S. | en_US |
dc.contributor.author | Stetson, P. S. | en_US |
dc.contributor.author | Wolfe, R. R. | en_US |
dc.date.accessioned | 2006-04-07T20:39:50Z | |
dc.date.available | 2006-04-07T20:39:50Z | |
dc.date.issued | 1989-11 | en_US |
dc.identifier.citation | Oldham, K. T., Guice, K. S., Stetson, P. S., Wolfe, R. R. (1989/11)."Bacteremia-induced suppression of alveolar surfactant production,." Journal of Surgical Research 47(5): 397-402. <http://hdl.handle.net/2027.42/27706> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WM6-4BNF2MK-22N/2/d616e05c903b22d293b950884fe46a40 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/27706 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2682003&dopt=citation | en_US |
dc.description.abstract | Sepsis is characterized by Adult Respiratory Distress Syndrome (ARDS)-like pulmonary dysfunction largely attributed to alveolar capillary endothelial cell injury which causes increased microvascular permeability and interstitial edema formation. In addition, quantitative and qualitative abnormalities of the pulmonary surfactant system may be important features of some clinical and experimental lung injuries. This study was designed to investigate the relationship of bacteremia and endotoxemia to pulmonary surfactant production in vivo. A technique for estimation of pulmonary surfactant phospholipid synthesis measuring incorporation of a stable isotope precursor ([2-13C]acetate) into dipalmitoylphosphatidylcholine (DPPC) in alveolar lavage fluid was developed. Male 350 g Sprague-Dawley rats had placement of central venous catheters. After overnight recovery, sublethal bacteremia (Escherichia coli, 1 x 108 organisms, iv) and sublethal endotoxemia (Difco; 10 mg/kg, iv) were induced. Both were associated with lung microvascular permeability increases consistent with capillary endothelial injury. Eight-hour infusions of [2-13C]acetate were given. After sacrifice, bronchoalveolar washings and lung tissue were obtained and [2-13C] incorporation into lavage and lung DPPC was measured by gas chromatography mass spectroscopy. Endotoxin-treated animals had a 21.5% reduction in label incorporation into DPPC [1.215 +/- 0.145 APE (%) sham versus 0.954 +/- 0.144 APE (%) experimental, P> 0.05] and bacteremic animals had a 56.9% diminution of [2-13C]acetate incorporation [1.215 +/- 0.145 APE (%) sham versus 0.524 +/- 0.56 APE (%) experimental, P < 0.05]. Bacteremia-induced dysfunction of alveolar type II epithelial cells manifested as diminished alveolar surfactant phospholipid production may be a contributing factor to sepsis-induced respiratory failure. | en_US |
dc.format.extent | 785298 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Bacteremia-induced suppression of alveolar surfactant production, | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Surgery and Anesthesiology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Section of Pediatric Surgery, Upjohn Center for Clinical Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109-0245, USA | en_US |
dc.contributor.affiliationum | Section of General Surgery, Department of Surgery, Upjohn Center for Clinical Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109-0245, USA | en_US |
dc.contributor.affiliationum | Department of Pharmacology, Upjohn Center for Clinical Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109-0245, USA | en_US |
dc.contributor.affiliationother | Shriner's Burns Institute, University of Texas Medical Branch, Galveston, Texas 77550, USA | en_US |
dc.identifier.pmid | 2682003 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/27706/1/0000092.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0022-4804(89)90090-5 | en_US |
dc.identifier.source | Journal of Surgical Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.