The colocalization of substance P and prodynorphin immunoreactivity in neurons of the medial preoptic area, bed nucleus of the stria terminalis and medial nucleus of the amygdala of the Syrian hamster
dc.contributor.author | Neal, Jr. , Charles R. | en_US |
dc.contributor.author | Swann, Jennifer M. | en_US |
dc.contributor.author | Newman, Sarah Winans | en_US |
dc.date.accessioned | 2006-04-07T20:41:53Z | |
dc.date.available | 2006-04-07T20:41:53Z | |
dc.date.issued | 1989-09-04 | en_US |
dc.identifier.citation | Neal, Jr., Charles R., Swann, Jennifer M., Newman, Sarah Winans (1989/09/04)."The colocalization of substance P and prodynorphin immunoreactivity in neurons of the medial preoptic area, bed nucleus of the stria terminalis and medial nucleus of the amygdala of the Syrian hamster." Brain Research 496(1-2): 1-13. <http://hdl.handle.net/2027.42/27761> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6SYR-4834KV3-1CR/2/e3312e7e33f80a649a232074a746a71c | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/27761 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2478255&dopt=citation | en_US |
dc.description.abstract | To determine the extent of colocalization of substance P (SP) and prodynorphin peptides within neurons of the medial nucleus of the amygdala (AMe), medial bed nucleus of the stria terminalis (BNSTm) and medial preoptic area (MPOA), we incubated colchicine-treated Syrian hamster brain tissue in an antiserum mixture containing rat anti-SP antibody combined with 1 of 3 rabbit antibodies against prodynorphin peptides: anti-dynorphin A(1-17), anti-dynorphin B(1-13) or anti-C-peptide. This was followed by incubation in a secondary antiserum mixture containing fluorescein-labelled anti-rabbit and rhodamine-labelled anti-rat antibodies. Sections were viewed with an epifluorescence microscope using blue light excitation for fluorescein and green light excitation for rhodamine. Colocalization of SP and prodynorphin labelling was observed in neurons of the caudal parts of AMe, BNSTm and MPOA, areas which are essential for male mating behavior. The colocalization was most extensive in the dorsolateral part of the caudal MPOA, the caudodorsal part of the BNSTm, and in the posterodorsal subdivision of AMe. Although all 3 dynorphin peptides coexisted with SP in these areas, dynorphin B did so less than C-peptide, and dynorphin A less than dynorphin B. | en_US |
dc.format.extent | 1061635 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | The colocalization of substance P and prodynorphin immunoreactivity in neurons of the medial preoptic area, bed nucleus of the stria terminalis and medial nucleus of the amygdala of the Syrian hamster | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor, MI 48109-0616, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor, MI 48109-0616, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Biological Sciences, Rutgers University, Newark, NJ 07102, U.S.A. | en_US |
dc.identifier.pmid | 2478255 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/27761/1/0000154.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-8993(89)91046-9 | en_US |
dc.identifier.source | Brain Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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